Potential role of mitogen-activated protein kinase during meiosis resumption in bovine oocytes

Biology of Reproduction
R A FissoreG F Vande Woude

Abstract

During meiotic maturation, numerous cytoplasmic and nuclear events take place that prepare the oocytes for fertilization. These changes are initiated by an increase in the activity of several kinases, most notably maturation-promoting factor, also called histone H1 kinase. Another kinase, mitogen-activated protein (MAP) kinase, is also stimulated during this period. In this study, we investigated the role of MAP kinase in bovine oocyte maturation. First, the kinetics of activation of histone H1 and MAP kinases during maturation were assessed simultaneously by evaluating their catalytic activities in vitro. We found that they are activated at approximately the same time, around germinal vesicle breakdown (GVBD). Then, at approximately 15 h of maturation, the activity of H1 kinase temporarily decreases, whereas MAP kinase remains high through the metaphase II stage. Second, the activation and catalytic activity of MAP kinase was directly evaluated by Western blotting and by an in-gel kinase assay. We determined that MAP kinase becomes activated and exhibits a decreased mobility through SDS-polyacrylamide gels, and that its catalytic activity increases as maturation progresses. In our system, most of the MAP kinase activity can be...Continue Reading

Citations

Dec 24, 1997·Molecular Reproduction and Development·H WuR A Fissore
Sep 18, 1998·Molecular Reproduction and Development·S HaraguchiE Sato
Aug 24, 2000·Molecular Reproduction and Development·N YoshidaM Yamashita
Aug 31, 2002·Molecular Reproduction and Development·M E Dell'AquilaP Minoia
Oct 30, 2004·Molecular Reproduction and Development·T L Smythe, S A Stricker
Dec 25, 2002·Theriogenology·T Miyano
Sep 14, 2001·Reproduction in Domestic Animals = Zuchthygiene·B MeineckeS Meinecke-Tillmann
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·James HarperElizabeth J Robertson
Dec 19, 2008·Cloning and Stem Cells·Fuliang DuY Eugene Chen
Sep 14, 2010·Human Reproduction Update·Stéphane Brunet, Marie Hélène Verlhac
Nov 3, 2009·Molecular Human Reproduction·Fuller W BazerKayla Bayless
Oct 23, 2002·Biology of Reproduction·Yasuhiko NakamuraHiroshi Kato
Jan 21, 2003·Biology of Reproduction·Satoshi OhashiHideaki Tojo
Feb 28, 2003·Biology of Reproduction·Hyukjung KwonGuoyao Wu
Jan 23, 2004·Biology of Reproduction·Jon T SelfGuoyao Wu
Apr 30, 2004·Biology of Reproduction·Luis Gerardo Villa-Diaz, Takashi Miyano
Apr 16, 2004·Biology of Reproduction·Amy Inselman, Mary Ann Handel
Dec 4, 2004·Biology of Reproduction·Guoyao WuThomas E Spencer
Mar 30, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Manabu KurokawaRafael A Fissore
Jun 6, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Teresa De SantisMaria Elena Dell'Aquila
Oct 14, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·L A RispoliJ L Edwards
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Korakot NganvongpanitDawit Tesfaye
May 21, 2013·Zygote : the Biology of Gametes and Early Embryos·Paulo Roberto Antunes RosaPaulo Bayard Dias Gonçalves
Jun 30, 2015·The Journal of Reproduction and Development·Leah M HooperJ Lannett Edwards
Apr 21, 2009·Animal Reproduction Science·Mithat EvecenSerhat Pabuccuoğlu
Feb 18, 2011·Reproduction in Domestic Animals = Zuchthygiene·E DovolouD Rizos
Jun 26, 2010·Molecular Reproduction and Development·Stephen M Downs
Apr 2, 2014·Molecular Reproduction and Development·Xianhong MoYunpeng Hou

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