Potential role of proprotein convertase SKI-1 in the mineralization of primary bone.

Cells, Tissues, Organs
Jeff P GorskiN G Seidah

Abstract

The biochemical mechanism controlling nucleation of mineral crystals in developing bone, along with the growth and propagation of these crystals once formed, remains poorly understood. To define the nucleation mechanism, a proteomics analysis was begun on isolated biomineralization foci (BMF), sites of initial crystal nucleation in osteoblastic cell cultures and in primary bone. Comparative analyses of the protein profile for mineralized BMF with that for total osteoblast cultures revealed the latter were enriched in several proteins including BAG-75 and BSP, as well as fragments of each. When 12 protease inhibitors were added separately to UMR 106-01 osteoblastic cultures, only the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) blocked cleavage of BAG-75 and BSP, and prevented mineral crystal nucleation within BMF. Consideration of the specificities of the inhibitors tested and the fact that AEBSF inhibition was not dependent upon inclusion of FBS in the culture media indicated that mineral nucleation does not require serine protease plasmin, thrombin, kallikrein, urokinase, C1s or furin. In contrast, SKI-1 (S1P or site-1) is a membrane-bound serine protease inhibitable by AEBSF. We s...Continue Reading

References

Jan 1, 1980·Calcified Tissue International·D B Kimmel, W S Jee
Jan 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C H TurnerT Yoshikawa
Jan 1, 1996·Connective Tissue Research·H A GoldbergG K Hunter
May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·J P Gorski
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·N G SeidahM Marcinkiewicz
Feb 7, 2001·The Journal of Biological Chemistry·A WangR J Midura
Jan 5, 2002·The Journal of Biological Chemistry·Aram ElagozNabil G Seidah
Mar 15, 2003·The Journal of Biological Chemistry·Mat Leighton, Karl E Kadler
Oct 28, 2003·The Journal of Biological Chemistry·Barry M SteiglitzDaniel S Greenspan
Aug 18, 2006·Biological Chemistry·Nabil G SeidahAnnik Prat
Jul 12, 2007·The Journal of Biological Chemistry·Philomena PullikotilNabil G Seidah

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Citations

Apr 2, 2010·Endocrine Reviews·Xiang-Hong XuHong-Wen Deng
Dec 4, 2012·Viruses·Dominique J BurriAntonella Pasquato
Jan 15, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Wafa BouleftourLuc Malaval
Sep 30, 2009·Biochimica Et Biophysica Acta·Ellis E Golub
Jul 26, 2013·The Journal of Investigative Dermatology·Nina WeißMarkus Böhm
Dec 1, 2017·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Rebecca N JeromeJoshua C Denny
Aug 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Xinxin ChenLei Wang

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