Potential Role of the Formation of Tunneling Nanotubes in HIV-1 Spread in Macrophages

The Journal of Immunology : Official Journal of the American Association of Immunologists
Michihiro HashimotoShinya Suzu

Abstract

Tunneling nanotubes (TNTs), the long membrane extensions connecting distant cells, have emerged as a novel form of cell-to-cell communication. However, it is not fully understood how and to what extent TNTs contribute to intercellular spread of pathogens including HIV-1. In this study, we show that HIV-1 promotes TNT formation per se via its protein Nef and a cellular protein M-Sec, which appears to mediate approximately half of viral spread among monocyte-derived macrophages (MDMs). A small compound that inhibits M-Sec-induced TNT formation reduced HIV-1 production by almost half in MDMs. Such inhibition was not observed with Nef-deficient mutant HIV-1 that fails to promote TNT formation and replicates less efficiently than the wild-type HIV-1 in MDMs. The TNT inhibitor-sensitive/Nef-promoting viral production was also observed in a T cell line ectopically expressing M-Sec, but not in another M-Sec(-) T cell line. Our results suggest the importance of TNTs in HIV-1 spread among MDMs and might answer the long-standing question how Nef promotes HIV-1 production in a cell type-specific manner.

References

Jan 26, 1995·The New England Journal of Medicine·F KirchhoffR C Desrosiers
Apr 1, 1993·Journal of Virology·D S DimitrovM A Martin
May 9, 2000·Biochemical and Biophysical Research Communications·S YonezawaA Moriyama
Feb 14, 2004·Science·Amin RustomHans-Hermann Gerdes
Jul 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Björn OnfeltDaniel M Davis
Sep 20, 2005·Immunity·Simon C Watkins, Russell D Salter
Nov 3, 2006·Journal of Virology·Marion SourisseauOlivier Schwartz
Feb 13, 2007·Nature Cell Biology·Nathan M ShererWalther Mothes
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Irena KadiuHoward E Gendelman
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Holly R ChinneryPaul G McMenamin
Apr 24, 2008·Nature Reviews. Molecular Cell Biology·Daniel M Davis, Stefanie Sowinski
May 6, 2008·Current Opinion in Cell Biology·Hans-Hermann Gerdes, Raquel Negrão Carvalho
Sep 24, 2008·Retrovirology·John L Foster, J Victor Garcia
Feb 10, 2009·Nature Cell Biology·Karine GoussetChiara Zurzolo
May 29, 2009·Current Opinion in Cell Biology·Bing He, Wei Guo
Oct 28, 2009·Immunology and Cell Biology·Susan Moir, Anthony S Fauci
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Anne ChauveauDaniel M Davis
Jun 19, 2010·Developmental Biology·Christina PyrgakiLee Niswander
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xiang WangHans-Hermann Gerdes
Nov 3, 2010·Biochemical and Biophysical Research Communications·Kyoji HagiwaraYoko Aida
Nov 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Nancy Van ProoyenGenoveffa Franchini
May 14, 2011·Journal of Proteome Research·Irena Kadiu, Howard E Gendelman
Jul 27, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Irena Kadiu, Howard E Gendelman
Mar 13, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takashi ChiharaShinya Suzu
Dec 12, 2012·Cell Communication and Signaling : CCS·Libin Abraham, Oliver T Fackler
Dec 12, 2012·Cell and Tissue Research·Shunsuke KimuraHiroshi Ohno
Dec 15, 2012·Journal of Cell Science·Christian SchillerElisabeth H Weiss
Jun 20, 2013·Journal of Cell Science·Maddalena CostanzoChiara Zurzolo
Jul 23, 2013·Cell Death & Disease·N RainyY Kloog
Jul 28, 2013·Journal of Cell Science·Karine GoussetChiara Zurzolo
Sep 11, 2013·Bioscience, Biotechnology, and Biochemistry·Yuichi NakajimaMakoto Kimura
Apr 22, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Abu OsmanShinya Suzu
Dec 31, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Colleen R ZaccardRobbie B Mailliard
Jan 13, 2015·Cell Death and Differentiation·X Wang, H-H Gerdes

❮ Previous
Next ❯

Citations

Mar 26, 2016·Frontiers in Microbiology·Kenneth ChangVaibhav Tiwari
Feb 6, 2018·Annual Review of Immunology·Marthe F S Lindenbergh, Willem Stoorvogel
Feb 10, 2018·Frontiers in Immunology·Maeva DupontChristel Vérollet
Sep 20, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Soroor FarahnakJames G Martin
Mar 2, 2018·Journal of Virology·Mirosława PanasiukKrystyna Bieńkowska-Szewczyk
Aug 4, 2018·Annual Review of Cell and Developmental Biology·Yukiko M YamashitaMichael Buszczak
Sep 13, 2018·Journal of Cellular Physiology·Rahul MittalVasanti M Jhaveri
Mar 17, 2019·The Journal of Biological Chemistry·Rajaiah PerguSivaram V S Mylavarapu
Feb 7, 2020·Journal of Virology·Robert J J JansensHerman W Favoreel
Apr 9, 2020·Molecular Biology of the Cell·Casey J LatarioHenry N Higgs
Aug 5, 2017·Frontiers in Molecular Biosciences·Muhammad Nawaz, Farah Fatima
Mar 9, 2018·Frontiers in Immunology·Lucie BracqJérôme Bouchet
Apr 27, 2018·International Journal of Molecular Sciences·Silvana ValdebenitoEliseo Eugenin
Nov 18, 2018·Journal of Cell Communication and Signaling·Jaime UhlKarine Gousset
Dec 18, 2018·Frontiers in Pharmacology·Dominik D KaltenbachVaibhav Tiwari
Nov 5, 2019·Frontiers in Immunology·Michael H LehmannVolker Erfle
Nov 10, 2018·Cell Communication and Signaling : CCS·Eshna JashSeema Sehrawat
Jun 27, 2019·Cells·Mitja DrabAleš Iglič
Dec 20, 2019·Retrovirology·Sarkis SarkisCynthia A Pise-Masison
May 2, 2020·Viruses·Maeva Dupont, Quentin James Sattentau
Mar 6, 2018·Frontiers in Cell and Developmental Biology·Dacheng Liang
Jul 30, 2018·Retrovirology·Jérémy DuflooOlivier Schwartz
Apr 18, 2019·MBio·Soraya MezouarJean-Louis Mege
May 12, 2019·The Journal of Cell Biology·Manish Sharma, Srinivasa Subramaniam
Apr 3, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Srinivasa Subramaniam
Apr 7, 2019·Scientific Reports·Aysegul Dilsizoglu SenolChiara Zurzolo
Oct 8, 2020·Nature Reviews. Microbiology·Jennifer E JonesSeema S Lakdawala
Nov 24, 2020·Platelets·Yvonne BaumerLishomwa C Ndhlovu
Nov 25, 2020·AIDS·Kelly HughesMaria Blasi
Dec 29, 2020·Biochemical Society Transactions·Gal HaimovichJeffrey E Gerst
Mar 2, 2021·The EMBO Journal·Diégo Cordero Cervantes, Chiara Zurzolo
Mar 7, 2021·International Journal of Molecular Sciences·Xiaoning Han, Xiang Wang
Mar 13, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xiao-Tong WangYu-He Yuan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.