Potential route for fetal therapy: intramembranous absorption of intraamniotically injected furosemide

American Journal of Obstetrics and Gynecology
W M GilbertRobert A Brace

Abstract

Our purpose was to determine whether intraamniotically injected furosemide could be absorbed directly into fetal circulation by the ovine intramembranous pathway. Nine chronically catheterized fetal sheep, five with an intact and four with a surgically ligated esophagus, were studied for 7 hours on 2 consecutive days. Day 1 was a control day in which the fetal parameters of arterial, venous, and amniotic fluid pressures; heart rate; and urine flow were measured continuously. Fetal and maternal blood, amniotic fluid, and fetal urine were sampled for osmolality, electrolytes, blood gases, and pH twice during the first hour and hourly thereafter. On day 2, 15 mg of furosemide was injected into the amniotic cavity after 1 hour and fetuses were monitored in the same fashion as on the control day. On day 2, both intact and ligated fetuses demonstrated a rapid and prolonged increase in urine flow (p < 0.01) compared with control day fetuses. Esophageal ligation decreased, but did not eliminate, the diuresis. Free water clearance increased equally (p < 0.05) in both groups compared with control fetuses. Sodium and chloride excretion increased significantly in intact (sodium 591% +/- 220% and chloride 763% +/- 295%) and ligated fetuses ...Continue Reading

References

Mar 15, 1985·American Journal of Obstetrics and Gynecology·P F ChamberlainF A Manning
Jan 1, 1993·American Journal of Obstetrics and Gynecology·T F KellyRobert A Brace

Citations

Jun 1, 1997·Clinical Obstetrics and Gynecology·S J Kilpatrick
Oct 18, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jen-Ruey TangSteven H Abman
Jan 27, 2007·Placenta·M H BeallM G Ross
Apr 29, 2009·American Journal of Obstetrics and Gynecology·Katherine M GestelandRobert A Brace
Jan 23, 1999·American Journal of Obstetrics and Gynecology·Robert A BraceC Y Cheung
Apr 16, 1998·American Journal of Obstetrics and Gynecology·M A CurranM G Ross
Jul 16, 2005·American Journal of Obstetrics and Gynecology·Elizabeth A AdamsRobert A Brace
Jul 20, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert A BraceDebra F Anderson
Aug 1, 1997·The Anatomical Record·L ShandleyE M Wintour

Related Concepts

Process of Absorption
Structure of Amnion
Metazoa
Chloride Ion Level
Diuresis
Esophagus
Embryopathies
Extraembryonic Membranes
Fetal Structures
Salix - substance

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