Potential route for fetal therapy: intramembranous absorption of intraamniotically injected furosemide

American Journal of Obstetrics and Gynecology
W M GilbertRobert A Brace


Our purpose was to determine whether intraamniotically injected furosemide could be absorbed directly into fetal circulation by the ovine intramembranous pathway. Nine chronically catheterized fetal sheep, five with an intact and four with a surgically ligated esophagus, were studied for 7 hours on 2 consecutive days. Day 1 was a control day in which the fetal parameters of arterial, venous, and amniotic fluid pressures; heart rate; and urine flow were measured continuously. Fetal and maternal blood, amniotic fluid, and fetal urine were sampled for osmolality, electrolytes, blood gases, and pH twice during the first hour and hourly thereafter. On day 2, 15 mg of furosemide was injected into the amniotic cavity after 1 hour and fetuses were monitored in the same fashion as on the control day. On day 2, both intact and ligated fetuses demonstrated a rapid and prolonged increase in urine flow (p < 0.01) compared with control day fetuses. Esophageal ligation decreased, but did not eliminate, the diuresis. Free water clearance increased equally (p < 0.05) in both groups compared with control fetuses. Sodium and chloride excretion increased significantly in intact (sodium 591% +/- 220% and chloride 763% +/- 295%) and ligated fetuses ...Continue Reading


Mar 15, 1985·American Journal of Obstetrics and Gynecology·P F ChamberlainF A Manning
Jan 1, 1993·American Journal of Obstetrics and Gynecology·T F KellyRobert A Brace


Jun 1, 1997·Clinical Obstetrics and Gynecology·S J Kilpatrick
Oct 18, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jen-Ruey TangSteven H Abman
Jan 27, 2007·Placenta·M H BeallM G Ross
Apr 29, 2009·American Journal of Obstetrics and Gynecology·Katherine M GestelandRobert A Brace
Jan 23, 1999·American Journal of Obstetrics and Gynecology·Robert A BraceC Y Cheung
Apr 16, 1998·American Journal of Obstetrics and Gynecology·M A CurranM G Ross
Jul 16, 2005·American Journal of Obstetrics and Gynecology·Elizabeth A AdamsRobert A Brace
Jul 20, 2018·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert A BraceDebra F Anderson
Aug 1, 1997·The Anatomical Record·L ShandleyE M Wintour

Related Concepts

Process of Absorption
Structure of Amnion
Chloride Ion Level
Extraembryonic Membranes
Fetal Structures
Salix - substance

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.