PMID: 3761361Jan 1, 1986Paper

Potential-sensitive response mechanism of diS-C3-(5) in biological membranes

The Journal of Membrane Biology
G Cabrini, A S Verkman

Abstract

The potential-sensitive response mechanism of 3,3'-dipropylthiodicarbocyanine iodide (diS-C3-(5] was examined based on our previous model of diS-C3-(5) interaction with brush border membrane vesicles (BBMV) in the absence of a membrane potential. The model contained binding (6 msec), reorientation (30 msec), dimerization (less than 10 nsec), and translocation (1 sec) reaction steps (Cabrini & Verkman, 1986. J. Membrane Biol. 90:163-175). Transmembrane potentials (psi) were induced in BBMV by K+ gradients and valinomycin. Steady-state diS-C3-(5) fluorescence (excitation 622 nm, emission 670 nm) increased linearly with psi. The reorientation and translocation reaction steps were resolved by the stopped-flow technique as a biexponential decrease in fluorescence following mixture of diS-C3-(5) with BBMV at varying psi. The fractional amplitude of the faster exponential increased from 0.36 to 0.73 with increasing psi (-17 to 87 mV); the time constant for the faster exponential (30-35 msec) was independent of psi. There were single exponential kinetics (0.5-1.5 sec) for diS-C3-(5) fluorescence response to a rapid (less than 2 msec) change in psi in the absence of a diS-C3-(5) concentration gradient. These results, and similar finding...Continue Reading

References

Sep 27, 1979·Biochemical and Biophysical Research Communications·P I Lelkes
Jun 30, 1976·The Journal of Membrane Biology·A Waggoner
Jan 1, 1986·The Journal of Membrane Biology·G Cabrini, A S Verkman
Sep 1, 1974·The Biochemical Journal·A G Booth, A J Kenny
Jan 1, 1974·Methods in Enzymology·C Huang, T E Thompson
Jan 1, 1984·The Journal of Membrane Biology·R D GuntherE M Wright
May 1, 1983·Journal of Biochemical and Biophysical Methods·D A Barrow, B R Lentz

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Citations

Dec 6, 1989·Biochimica Et Biophysica Acta·O Eidelman, Z I Cabantchik
Oct 8, 1990·Biochimica Et Biophysica Acta·G Cevc
Jul 20, 2002·Archives of Biochemistry and Biophysics·Eider LarrarteJ Alfredo Martínez
Oct 1, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroaki SuzukiTsunenori Nozawa
Dec 22, 2004·Archives of Microbiology·Christian LaflammeCaroline Duchaine
Jan 30, 2009·The Plant Journal : for Cell and Molecular Biology·Sabine KuhnUte C Vothknecht
Apr 14, 2016·European Biophysics Journal : EBJ·Jose A Alvarez-Bustamante, Victor V Lemeshko
Dec 17, 2016·ACS Applied Materials & Interfaces·Jin GuPeng Yang
Feb 4, 2009·Journal of Applied Microbiology·A MaggeP Setlow
Mar 3, 2018·Scientific Reports·Naoki MiyazawaMamoru Mabuchi
Sep 5, 2003·Scandinavian Journal of Infectious Diseases·Magnus ArpiJørgen J Leisner
Nov 28, 2018·Molecular Diagnosis & Therapy·Giulio Cabrini
Mar 15, 1990·Biochimica Et Biophysica Acta·J C Smith
Nov 1, 1994·Biochimica Et Biophysica Acta·I Ahmed, G Krishnamoorthy
Feb 17, 1992·Biochimica Et Biophysica Acta·A ChattopadhyayB Raman

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