Potential urinary biomarkers of disease activity in Crohn's disease

Scandinavian Journal of Gastroenterology
Lajos MarkóIstván Wittmann

Abstract

Measurement of the immunoreactive urinary albumin (ir-uAlb) concentration by immunological methods was found to be an effective method to identify disease activity in Crohn's disease (CD). Recently a size-exclusion (SE) high performance liquid chromatography (HPLC) method was developed to measure both ir-uAlb and non-immunoreactive urinary albumin (total, t-uAlb). We aimed to follow-up one of our CD patients with frequent remissions and exacerbation phases comparing the changes of disease activity parameters and the concentration of ir-uAlb and t-uAlb. The surprising results led us to perform measurements in greater depth. Concentration of ir-uAlb was measured by immunoturbidimetry (IT) and t-uAlb by SE-HPLC. Albumin peak of SE-HPLC was collected and applied to a reversed-phase (RP) HPLC and to gel-electrophoresis. Eluted peaks of RP-HPLC and identified bands of gel-electrophoresis were analyzed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). The concentration of t-uAlb was 15 times higher than that of the ir-uAlb during active state. The RP-HPLC and the gel-electrophoresis separation proved that albumin peak by size-exclusion consists of three different peaks. MALDI-TOF/MS mea...Continue Reading

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Citations

Jun 29, 2012·Digestive Diseases and Sciences·Anna VaiopoulouGeorge Zografos
Nov 20, 2018·European Journal of Clinical Investigation·Balázs SzirmayTamás Kőszegi
Oct 12, 2018·Journal of Crohn's & Colitis·Javier P Gisbert, María Chaparro
Aug 30, 2011·Critical Reviews in Clinical Laboratory Sciences·Marijn M SpeeckaertJoris R Delanghe

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