Potential use of high-throughput sequencing of soil microbial communities for estimating the adverse effects of continuous cropping on ramie (Boehmeria nivea L. Gaud)

PloS One
Siyuan ZhuQiuzhong Dai

Abstract

Ramie (Boehmeria nivea L. Gaud) fiber, one of the most important natural fibers, is extracted from stem bark. Continuous cropping is the main obstacle to ramie stem growth and a major cause of reduced yields. Root-associated microbes play crucial roles in plant growth and health. In this study, we investigated differences between microbial communities in the soil of healthy and continuously cropped ramie plants, and sought to identify potential mechanisms whereby these communities could counteract the problems posed by continuous cropping. Paired-end Illumina MiSeq analysis of 16S rRNA and ITS gene amplicons was employed to study bacterial and fungal communities. Long-term monoculture of ramie significantly decreased fiber yields and altered soil microbial communities. Our findings revealed how microbial communities and functional diversity varied according to the planting year and plant health status. Soil bacterial diversity increased with the period of ramie monoculture, whereas no significant differences were observed for fungi. Sequence analyses revealed that Firmicutes, Proteobacteria, and Acidobacteria were the most abundant bacterial phyla. Firmicutes abundance decreased with the period of ramie monoculture and correlat...Continue Reading

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Citations

Jul 6, 2021·Frontiers in Microbiology·Luigi OrrùFlavia Pinzari

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Datasets Mentioned

BETA
SRP110777
SRP110558

Methods Mentioned

BETA
electrophoresis
Illumina sequencing
PCR
Assay
PCA

Software Mentioned

custom
SPSS
Trimmomatic
Canoco
R scripts
Mothur
QIIME
FLASH
UPARSE

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