Potential use of superparamagnetic iron oxide nanoparticles for in vitro and in vivo bioimaging of human myoblasts

Scientific Reports
Kamil R WierzbinskiMaciej K Kurpisz

Abstract

Myocardial infarction (MI) is one of the most frequent causes of death in industrialized countries. Stem cells therapy seems to be very promising for regenerative medicine. Skeletal myoblasts transplantation into postinfarction scar has been shown to be effective in the failing heart but shows limitations such, e.g. cell retention and survival. We synthesized and investigated superparamagnetic iron oxide nanoparticles (SPIONs) as an agent for direct cell labeling, which can be used for stem cells imaging. High quality, monodisperse and biocompatible DMSA-coated SPIONs were obtained with thermal decomposition and subsequent ligand exchange reaction. SPIONs' presence within myoblasts was confirmed by Prussian Blue staining and inductively coupled plasma mass spectrometry (ICP-MS). SPIONs' influence on tested cells was studied by their proliferation, ageing, differentiation potential and ROS production. Cytotoxicity of obtained nanoparticles and myoblast associated apoptosis were also tested, as well as iron-related and coating-related genes expression. We examined SPIONs' impact on overexpression of two pro-angiogenic factors introduced via myoblast electroporation method. Proposed SPION-labeling was sufficient to visualize firef...Continue Reading

References

Jan 1, 1989·AJR. American Journal of Roentgenology·R WeisslederJ Lewis
Apr 8, 2003·Journal of the American College of Cardiology·Philippe MenaschéDenis Duboc
Dec 5, 2003·Nucleic Acids Research·Xiaowei Wang, Brian Seed
Nov 5, 2004·NMR in Biomedicine·Jeff W M Bulte, Dara L Kraitchman
May 15, 2007·Progress in Cardiovascular Diseases·Kozo HoshinoRoger J Hajjar
Aug 23, 2007·Environmental Science & Technology·Wassana YantaseeMarvin G Warner
Jan 12, 2008·Journal of Molecular and Cellular Cardiology·Philippe Menasché
Feb 22, 2008·Nature·Vincent F M Segers, Richard T Lee
Apr 4, 2008·Annals of Oncology : Official Journal of the European Society for Medical Oncology·T M KimUNKNOWN Lymphoma Subcommittee of the Korean Cancer Study Group
Oct 2, 2008·Nature Reviews. Drug Discovery·Siva LavuPhilip D Lambert
Nov 11, 2008·Mechanisms of Ageing and Development·Baruch BulvikMordechai Mottie Chevion
Nov 19, 2008·Nature Clinical Practice. Oncology·Shahriar S YaghoubiSanjiv S Gambhir
Apr 2, 2009·Current Opinion in Organ Transplantation·Damien J LaParZequan Yang
Jun 17, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ryan D RutledgeMarvin G Warner
Mar 6, 2012·Materials Today·Zachary R StephenMiqin Zhang
Apr 18, 2013·PloS One·Lorena Fernandes ArrudaEgle Machado de Almeida Siqueira
Dec 4, 2013·Methodist DeBakey Cardiovascular Journal·Robert E Michler
Dec 20, 2014·Chemical Research in Toxicology·Jinglu ZouJinke Wang
Jul 15, 2015·International Journal of Nanomedicine·Ralf P FriedrichChristoph Alexiou
Jan 20, 2016·Stem Cells International·Xiaofei LiJianjun Guan
Apr 5, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Xinpei MaoHonggang Cui
Jun 3, 2016·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Y LvL Zhang
Oct 28, 2016·Heart Failure Reviews·Peisen HuangYuejin Yang
Aug 23, 2016·International Journal of Molecular Sciences·Greta JarockyteRicardas Rotomskis
Aug 28, 2016·Current Treatment Options in Cardiovascular Medicine·Yen-Wen LiuPatrick C H Hsieh

❮ Previous
Next ❯

Citations

Oct 1, 2019·Advanced Materials·Byungji KimMichael J Sailor
Jun 12, 2020·Biomimetics·Maria P Nikolova, Murthy S Chavali
Oct 19, 2019·IET Nanobiotechnology·Alireza Allafchian, Seyed Sajjad Hosseini
Jan 26, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Martín E VillanuevaRaquel V Vico
May 22, 2021·Nanomedicine·Senem ÇitoğluNguyen Thi Kim Thanh
Jul 3, 2021·Pharmaceutics·Deanna D StueberVicki L Colvin
Aug 21, 2020·Current Topics in Medicinal Chemistry·Simon N MbuguaMartin O Onani
Oct 22, 2019·Molecular Pharmaceutics·Cátia DominguesAna Figueiras

❮ Previous
Next ❯

Methods Mentioned

BETA
Infrared Spectroscopy
NMR
PCR
Nuclear Magnetic Resonance
Assay
electrophoresis

Software Mentioned

VectorBuilder
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Aging Genetics (Keystone)

This feed focuses on aging epidemiology and genetic, epigenetic, and proteomic aspects underlying aging, as well as aging- associated biomarkers. Here the latest research in this domain.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.