Abstract
This paper describes a new attempt to enhance the production of antibody by delivery of an antigen to phagocytic antigen-presenting cells (e.g. macrophages) using gelatin microspheres. A model protein antigen, human gamma globulin (HGG), was incorporated into microspheres composed of gelatin which have an opsonic ability for macrophage phagocytosis. Subcutaneous injection of the microspheres induced the production of HGG-specific IgG antibody in the mouse serum to a great extent compared with that of HGG in soluble form or in Freund's incomplete adjuvant (FIA) form. There was an optimal concentration of cross-linking agent (glutaraldehyde) for the highest production of antibody. When gelatin microspheres were cross-linked at lower concentrations of glutaraldehyde, they were more extensively swollen in an aqueous solution, leading to an increase in the size of hydrated microspheres because of their lower cross-linking densities. The increased size of microspheres caused a decrease in their macrophage phagocytosis, whereas the release rate of HGG from the microspheres increased as the concentration of cross-linking agent became low. The balance of the two factors, the microsphere susceptibility to macrophage phagocytosis and the ...Continue Reading
References
Aug 1, 1976·Journal of Pharmaceutical Sciences·S L NailS L Hem
Aug 1, 1976·Journal of Pharmaceutical Sciences·S L NailS L Hem
Feb 1, 1992·Immunobiology·M T Aguado, P H Lambert
May 1, 1991·Journal of Pharmaceutical Sciences·P StjärnkvistI Sjöholm
Jan 1, 1990·The Journal of Pharmacy and Pharmacology·Y Tabata, Y Ikada
Jun 1, 1989·Vaccine·D T O'HaganS S Davis
Oct 1, 1989·Vaccine·D T O'HaganI Sjöholm
Feb 1, 1988·Vaccine·M E MartinJ F Newman
Jun 1, 1988·Vaccine·J KreuterP P Speiser
Jun 1, 1986·The Journal of Experimental Medicine·R J BenjaminH Waldmann
Jun 1, 1986·Vaccine·J KreuterP P Speiser
Jan 1, 1981·Journal of Immunological Methods·R G Titus, J M Chiller
Mar 1, 1993·The Journal of Pharmacy and Pharmacology·A J AlmeidaM R Brown
Jan 1, 1993·The Journal of Infectious Diseases·Z MoldoveanuJ Mestecky
May 28, 1993·Science·P A MarxJ H Eldridge
Citations
Feb 26, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Ajay Kumar GuptaAdam S G Curtis
May 4, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Isabella SchöllErika Jensen-Jarolim
Oct 19, 2002·Journal of Reproductive Immunology·Shari Lofthouse, Joanna Kemp
Aug 28, 1998·Trends in Biotechnology·D K Pettit, W R Gombotz
Jun 6, 2000·Advanced Drug Delivery Reviews·R K GuptaD T O'Hagan
Dec 1, 1996·Vaccine·R NakaokaY Ikada
Dec 31, 1997·Vaccine·E A LomotanP A Offita
Apr 8, 2011·Anti-cancer Drugs·Jitender MadanAnju Katyal
Jan 1, 1997·Microbiology and Immunology·H Kato, N Goto
May 5, 2010·World Journal of Gastroenterology : WJG·Yu MaXiao-Li Chen
Apr 9, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·James O HardinValeria T Milam
Dec 1, 1996·Journal of Pharmaceutical Sciences·Z Zhao, K W Leong
Aug 19, 2015·International Journal of Biological Macromolecules·Nityananda SahooKetousetuo Kuotsu
Aug 7, 2003·Expert Review of Vaccines·Elena Nechaeva
Dec 20, 2005·Biomaterials·Yong-Il ChungSoon Hong Yuk
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·G A SilvaR L Reis
Mar 1, 2011·Drug Delivery·M S SudheeshD V Kohli
Jan 10, 2018·Journal of Biomedical Materials Research. Part a·Ryosuke IwamaOsamu Suzuki
Mar 18, 2017·Dental Materials Journal·Koichi KidaYasutomo Yajima
Dec 12, 2020·Scientific Reports·C T PanY L Shiue
Oct 23, 2020·Polymers·Shamsul Bin SulaimanNg Min Hwei
May 1, 2021·Pharmaceutics·Tsu-Man ChiuNobutaka Hanagata