PMID: 7523662Jun 15, 1994Paper

Potentiation by ATP of the postsynaptic acetylcholine response at developing neuromuscular synapses in Xenopus cell cultures

The Journal of Physiology
W M Fu

Abstract

1. Extracellular application of ATP to developing Xenopus neuromuscular synapses in culture resulted in a marked increase in the amplitude and frequency of spontaneous synaptic currents, using whole-cell recording. 2. The postsynaptic action of ATP was examined by studying the response of isolated muscle cells to iontophoretically applied acetylcholine (ACh). ATP enhanced the responses of the muscle membrane to ACh. The order of potency for various nucleotides (ATP = ADP > AMP, adenosine, GTP) suggests that ATP acts through P2-purinoceptors. The effect of ATP on whole-cell currents was also abolished by the protein kinase inhibitor H-7. 3. Single-channel measurements indicate that ATP increased the mean open time of low-conductance ACh channels. No change in the conductance of ACh channels was observed. 4. Local application of ATP to one region of the elongated myocyte surface resulted in potentiated ACh responses only at the ATP-treated region, suggesting that the cytosolic second messengers were effectively confined within the muscle cytoplasm. 5. The results of the present study suggest that ATP released from the nerve terminals may potentiate the ACh response of developing muscle cells during the early phase of synaptogenes...Continue Reading

Citations

Jan 1, 1995·European Biophysics Journal : EBJ·R A GiniatullinF Vyskocil
Jan 12, 2011·Cellular and Molecular Life Sciences : CMLS·Geoffrey Burnstock, Henning Ulrich
May 21, 2015·Purinergic Signalling·Geoffrey Burnstock, Nicholas Dale
Jul 1, 1996·British Journal of Pharmacology·E MartíC Solsona
Mar 28, 2001·The Annals of Otology, Rhinology, and Laryngology·J L KreindlerP A Wackym
Sep 1, 1995·Progress in Neurobiology·W M Fu
Sep 10, 2020·International Journal of Molecular Sciences·Ayrat U ZiganshinSergey N Grishin

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