Potentiation by WIN 55,212-2 of GABA-activated currents in rat trigeminal ganglion neurones.

British Journal of Pharmacology
Zhi-Wang LiWang-Ping Hu

Abstract

Although both natural and synthetic cannabinoid compounds have been shown to exert an antinociceptive effect on acute and persistent pain, the anatomical locus of the target of cannabinoid-induced analgesia has not been fully elucidated. Here, we investigated the effects of the cannabinoid agonist WIN 55,212-2 on GABA-activated currents (I(GABA)) in rat primary sensory neurones. In the present study, experiments were performed on neurones freshly isolated from rat trigeminal ganglion (TG) by using whole-cell patch clamp and repatch techniques. GABA-evoked inward currents were potentiated by pretreatment with WIN 55,212-2 in a concentration-dependent manner (10(-10)-10(-8) M). WIN 55,212-2 shifted the GABA concentration-response curve upwards, with an increase of 30.3 +/- 3.7% in the maximal current response but with no significant change in the EC(50) (agonist concentration producing a half-maximal response) value. WIN 55,212-2 potentiated the responses to GABA in a manner independent of holding potential and in the absence of any change in the reversal potential of the current. This potentiation of I(GABA) induced by WIN 55,212-2 was almost completely blocked by AM 251 (3 x 10(-8) M), a CB(1) receptor antagonist, and, using th...Continue Reading

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Nov 18, 2009·British Journal of Pharmacology·Zhi-Wang LiWang-Ping Hu

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Citations

Aug 31, 2010·Neuropharmacology·Yan-Cai XiongWang-Ping Hu
Aug 26, 2014·The International Journal of Biochemistry & Cell Biology·Patrick McDonoughEric J Downer
Aug 21, 2010·The Journal of Physiology·Yu-Wei LiuJiang-Hong Ye
Nov 18, 2009·British Journal of Pharmacology·Zhi-Wang LiWang-Ping Hu
Feb 6, 2019·Biomolecules·Nadia R ZgajnarMario D Galigniana

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