Potentiation of antifungal activity of amphotericin B by azithromycin against Aspergillus species

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
M H NguyenA S Lewin

Abstract

The potential role of azithromycin in combination with amphotericin B against 25 clinical isolates of Aspergillus was assessed. The MIC of amphotericin B was 1 microg/ml for 44% of the isolates, 0.5 microg/ml for 48%, and 0.25 microg/ml for 8%. All isolates were resistant to azithromycin. Synergism, defined as a > or = twofold reduction in the MIC of both drugs upon combination, was demonstrated between amphotericin B and azithromycin for all 25 isolates. To prove that azithromycin exerts its antifungal effect by inhibiting protein synthesis, we studied [35S]-methionine incorporation into protein in one Aspergillus isolate. Neither amphotericin B at 0.125 microg/ml (fourfold below its MIC) nor azithromycin at 16 microg/ml (> or = 16-fold below its MIC) had any effect on protein synthesis when tested alone. Upon combination, however, a 68% inhibition in protein synthesis was evident by the inhibition of [35S]-methionine incorporation.

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Citations

May 10, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jose A Vazquez
May 4, 2011·Antimicrobial Agents and Chemotherapy·Erico Silva LoretoJanio Morais Santurio
Sep 18, 2002·Antimicrobial Agents and Chemotherapy·Javier AfeltraPaul E Verweij
Sep 18, 2004·The Journal of Antimicrobial Chemotherapy·Manuel Cuenca-Estrella
Apr 9, 1999·Clinical Microbiology Reviews·J P Latgé
Jun 22, 2021·Applied Microbiology and Biotechnology·Qian ZhangZhangyong Song

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Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.