Potentiation of complement regulator factor H protects human endothelial cells from complement attack in aHUS sera.

Blood Advances
R B PouwD Wouters

Abstract

Mutations in the gene encoding for complement regulator factor H (FH) severely disrupt its normal function to protect human cells from unwanted complement activation, resulting in diseases such as atypical hemolytic uremic syndrome (aHUS). aHUS presents with severe hemolytic anemia, thrombocytopenia, and renal disease, leading to end-stage renal failure. Treatment of severe complement-mediated disease, such as aHUS, by inhibiting the terminal complement pathway, has proven to be successful but at the same time fails to preserve the protective role of complement against pathogens. To improve complement regulation on human cells without interfering with antimicrobial activity, we identified an anti-FH monoclonal antibody (mAb) that induced increased FH-mediated protection of primary human endothelial cells from complement, while preserving the complement-mediated killing of bacteria. Moreover, this FH-activating mAb restored complement regulation in sera from aHUS patients carrying various heterozygous mutations in FH known to impair FH function and dysregulate complement activation. Our data suggest that FH normally circulates in a less active conformation and can become more active, allowing enhanced complement regulation on hu...Continue Reading

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Aug 28, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gillian DekkersIlse Jongerius
Apr 20, 2021·Frontiers in Immunology·Felix PoppelaarsUNKNOWN SciFiMed consortium
Jul 1, 2021·Seminars in Immunopathology·M JalinkI Jongerius

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