PMID: 3759567Aug 1, 1986Paper

Potentiation of in vitro cytotoxic effects of misonidazole on human colon tumor cells by the differentiation-inducing agent N-methylformamide

International Journal of Radiation Oncology, Biology, Physics
C M ArundelA S Glicksman

Abstract

Human colon tumor cells (clone A) were studied in vitro with regard to modification of dose-dependent cytotoxicity to misonidazole (MISO) treatment by pre-exposure growth in medium containing the differentiation-inducing agent N-methylformamide (NMF). Cells were grown as exponential cultures and were exposed for 2 passages to 170 mM NMF before exposure to graded doses of MISO (0-100 mM, 3 hours at 37 degrees C, oxic or hypoxic). Both oxic and hypoxic cells could be sensitized to MISO cell killing. Using the 10% level of survival for comparison, the calculated MISO doses (mM) were: 105, 37, 50, and 10 for oxic control cells, hypoxic control cells, oxic-NMF treated cells, and hypoxic-NMF treated cells, respectively. Therefore, for NMF treated oxic cells, cell killing was increased by a factor of about 2.1, while for NMF treated hypoxic cells, cell killing increased by a factor of about 3.7. These data indicate that NMF treatment, while potentiating effects on both oxic and hypoxic cells, appears to have selectivity towards hypoxic cells. NMF may therefore have use in combined modality radiation therapy of solid tumors with electron-affinic radiosensitizers.

References

May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S J CollinsR C Gallo
Nov 1, 1985·International Journal of Radiation Oncology, Biology, Physics·J T LeithA S Glicksman
Feb 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C FriendT Sato
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Jul 31, 1984·Biochemical and Biophysical Research Communications·R F Cordeiro, T M Savarese
Jun 1, 1984·British Journal of Cancer·D W SiemannK T Wheeler
Jul 1, 1981·European Journal of Cancer & Clinical Oncology·K Sakamoto, S Aritake
Jun 1, 1982·British Journal of Cancer·A GescherG Atassi

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Citations

Apr 1, 1987·International Journal of Radiation Oncology, Biology, Physics·C M Arundel, J T Leith

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