PMID: 8610094Apr 2, 1996Paper

Potentiation of proton transfer function by electrostatic interactions in photosynthetic reaction centers from Rhodobacter sphaeroides: First results from site-directed mutation of the H subunit

Proceedings of the National Academy of Sciences of the United States of America
E Takahashi, C A Wraight

Abstract

The x-ray crystallographic structure of the photosynthetic reaction center (RC) has proven critical in understanding biological electron transfer processes. By contrast, understanding of intraprotein proton transfer is easily lost in the immense richness of the details. In the RC of Rhodobacter (Rb.) sphaeroides, the secondary quinone (QB) is surrounded by amino acid residues of the L subunit and some buried water molecules, with M- and H-subunit residues also close by. The effects of site-directed mutagenesis upon RC turnover and quinone function have implicated several L-subunit residues in proton delivery to QB, although some species differences exist. In wild-type Rb. sphaeroides, Glu L212 and Asp L213 represent an inner shell of residues of particular importance in proton transfer to QB. Asp L213 is crucial for delivery of the first proton, coupled to transfer of the second electron, while Glu L212, possibly together with Asp L213, is necessary for delivery of the second proton, after the second electron transfer. We report here the first study, by site-directed mutagenesis, of the role of the H subunit in QB function. Glu H173, one of a cluster of strongly interacting residues near QB, including Asp L213, was altered to G...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·M Y Okamura, G Feher
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M L PaddockM Y Okamura
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J P AllenD C Rees
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D K HansonM Schiffer
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·P MarótiP Sebban

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Citations

May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M L PaddockM Y Okamura
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·M L PaddockM Y Okamura
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·J MiksovskaP Sebban
Jul 24, 2015·Photosynthesis Research· GovindjeeDonald R Ort
Aug 29, 2006·Biochimica Et Biophysica Acta·Colin A Wraight
Jan 8, 2005·The Journal of Biological Chemistry·Hiroshi Ishikita, Ernst-Walter Knapp
Dec 16, 2005·The Journal of Biological Chemistry·Eiji Takahashi, Colin A Wraight
Jun 6, 2009·The Journal of Physical Chemistry. B·Yu KanekoIwao Ohmine

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