Potentiation of the antitumor effect of Merocyanine 540-mediated photodynamic therapy by amifostine and amphotericin B.

Photochemistry and Photobiology
Ichiro TsujinoFritz Sieber

Abstract

Leukemia and lymphoma cells are much more sensitive to Merocyanine 540 (MC540)-mediated photodynamic therapy (PDT) than normal pluripotent hematopoietic stem cells and normal colony forming unit-granulocyte/macrophage progenitors (CFU-GM). By contrast, most solid tumor cells are only moderately sensitive to MC540-PDT. The limited activity against solid tumor cells has detracted from MC540's appeal as a broad-spectrum purging agent. We report here that noncytotoxic concentrations of amifostine (Ethyol, Ethiofos, WR-2721) and amphotericin B used either alone or in combination potentiate the MC540-sensitized photoinactivation of leukemia cells, wild-type small cell lung cancer cells and cisplatin-resistant small cell lung cancer cells. Amphotericin B also enhances the MC540-sensitized photoinactivation of normal CFU-GM, whereas amifostine protects CFU-GM against the cytotoxic action of MC540-PDT. The yield of CD34-positive normal hematopoietic stem and progenitor cells is only minimally diminished by pretreatment with amifostine, amphotericin B or combinations of amifostine plus amphotericin B. Purging protocols that combine MC540-PDT with amifostine or with amifostine plus amphotericin B could offer a simple and effective approac...Continue Reading

References

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Citations

Feb 2, 2010·Bone Marrow Transplantation·B ČunderlíkováQ Peng
Nov 9, 2010·Bone Marrow Transplantation·B ČunderlíkováQ Peng
Apr 8, 2014·Journal of Photochemistry and Photobiology. B, Biology·L GándaraA Casas
Apr 5, 2011·International Journal of Laboratory Hematology·H HuangY Wu
Jun 9, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·E AlarcónJ C Scaiano
Mar 6, 2013·Materials·Alexandra B Ormond, Harold S Freeman
Jul 8, 2017·Journal of Chemical Biology·Farzaneh TavangarJahanbakhsh Asadi

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Methods Mentioned

BETA
flow cytometry
fluorescence spectroscopy

Software Mentioned

CellQuest

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