PMID: 7033798Nov 1, 1981Paper

Potentiation of the insulin-releasing capacity of tolbutamide by thiols: studies on the isolated perfused pancreas

Naunyn-Schmiedeberg's Archives of Pharmacology
H P Ammon, M Abdel-hamid

Abstract

It has been suggested that the islet thiol redox status plays a role in the regulation of beta-cell sensitivity in response to insulin secretagogues. Employing the isolated perfused rat pancreas, the effect of reduced glutathione (1 mM) and L-cysteine (5 mM) on insulin release induced by tolbutamide (0.2 mg/ml), glucose (5.6 and 11.1 mM) and tolbutamide (0.1 mg/ml) in the presence of 5.6 mM glucose was studied. In the absence of glucose or in the presence of 5.6 mM of glucose neither glutathione nor L-cysteine stimulated the release of insulin. Reduced glutathione potentiated the secretion induced by glucose (11.1 mM) during the first and the second phase. L-Cysteine potentiated only the first phase of glucose-induced insulin release, whereas the second phase was depressed. Both of the tested thiols potentiated the insulin secretory action of either tolbutamide (0.2 mg/ml) alone or tolbutamide (01. mg/ml) in the presence of glucose (5.6 mM). The data suggest that supplementation of thiols to the pancreatic beta-cells perse cannot initiate the insulin secretory process. It is also suggested that GSH and L-cysteine increase the sensitivity of beta-cells to the stimulatory action of tolbutamide and/or glucose.

References

May 10, 1979·Biochimica Et Biophysica Acta·S P Mukherjee, W S Lynn
Nov 2, 1979·Biochimica Et Biophysica Acta·C W HaestB Deuticke
Dec 14, 1978·Biochemical and Biophysical Research Communications·A BabaH Iwata
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Feb 1, 1972·Metabolism: Clinical and Experimental·G S LeveyD H Mintz
Sep 1, 1968·Endocrinology·D L CurryG M Grodsky
Oct 1, 1980·Diabetes·H P AmmonI Hagenloh
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN

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Citations

Sep 1, 1984·Naunyn-Schmiedeberg's Archives of Pharmacology·H P AmmonT Pfäffle
Nov 19, 1997·Journal of Endocrinological Investigation·D KonukoğluT Akçay
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Daiki NakatsuMasayuki Murata
Jul 1, 1985·Cell Biochemistry and Function·H P Ammon, M Mark

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