PMID: 11331222May 2, 2001Paper

Potentiation response of cultured human uterine leiomyoma cells to various growth factors by endothelin-1: role of protein kinase C

European Journal of Endocrinology
Isabelle EudeMichelle Breuiller-Fouché

Abstract

Factors responsible for the abnormal proliferation of myometrial cells that accompanies leiomyoma formation are unknown, although steroid hormones and peptide growth factors have been implicated. We hypothesized that endothelin-1 (ET-1) is a physiological regulator of tumor growth. In this study, we investigated the role of ET-1 on growth of human leiomyoma cells and its synergistic effect with growth factors, as well as the signaling pathway involved in this interaction. Leiomyoma cell proliferation was assayed by [H]thymidine incorporation and cell number. Protein kinase C (PKC) isoforms were analyzed by Western blot using specific antibodies. ET-1 on its own was unable to stimulate DNA synthesis but potentiated the leiomyoma cell growth effects of basic fibroblast growth factor (bFGF), epidermal growth factor (EGF), IGF-I and IGF-II. The failure of a protein tyrosine kinase (PTK) inhibitor, tyrphostin 51, to affect the potentiating effect of ET-1, supports the hypothesis of non-involvement of PTK in this process. The inhibition of PKC by calphostin C or its down-regulation by phorbol 12,13-dibutyrate (PDB) eliminated the potentiating effect of ET-1, but did not block cell proliferation induced by the growth factors alone. Fi...Continue Reading

Citations

Jun 7, 2013·Human Reproduction·Pietro SantulliCharles Chapron
Apr 13, 2005·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·T-K LeeC-H Kim
Aug 21, 2013·PloS One·Pietro SantulliDidier Borderie
Sep 23, 2014·American Journal of Hypertension·Yentl C HaanLizzy M Brewster
May 4, 2012·Biology of Reproduction·Zahra Tanfin, Michelle Breuiller-Fouché
Dec 26, 2001·Biology of Reproduction·Isabelle EudeMichelle Breuiller-Fouché
Dec 3, 2003·American Journal of Physiology. Cell Physiology·Philippe RobinDenis Leiber

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