Potentiometric Adsorption Isotherm Analysis of a Molecularly Imprinted Polymer Interface for Small-Biomolecule Recognition

ACS Omega
Shoichi Nishitani, Toshiya Sakata

Abstract

In this paper, we report a direct and quantitative analytical method of small-biomolecule recognition with a molecularly imprinted polymer (MIP) interface, taking advantage of the potentiometric principle of a field-effect transistor (FET) sensor, which enables the direct detection of ionic charges without using labeling materials such as fluorescent dyes. The interaction of low-molecular-weight oligosaccharides such as paromomycin and kanamycin with the MIP interface including phenylboronic acid (PBA) was directly and quantitatively analyzed from the electrical signals of an MIP-coated FET sensor. In particular, the change in the potential response of the FET sensor was derived on the basis of the multi-Langmuir adsorption isotherm equations, considering the change in the molecular charges of PBA caused by the adsorption equilibrium of the analytes with the vinyl PBA-copolymerized MIP membrane. Thus, the potentiometric adsorption isotherm analysis can elucidate the formation of selective binding sites at the MIP interface. The electrochemical analysis of the functional biointerface used in this study supports the design and construction of sensors for small biomarkers.

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Citations

Oct 9, 2019·Science and Technology of Advanced Materials·Kensuke ItoToshiya Sakata
Apr 13, 2021·ACS Sensors·George T WilliamsJohn S Fossey
Apr 26, 2021·Biosensors & Bioelectronics·Hassan Karimi-MalehAmani Al-Othman
Jan 16, 2019·ACS Applied Materials & Interfaces·Shoichi Nishitani, Toshiya Sakata
Sep 21, 2018·ACS Applied Materials & Interfaces·Taira Kajisa, Toshiya Sakata
May 13, 2019··Toshiya Sakata, Toshiya Sakata

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Methods Mentioned

BETA
biosensors
biosensing
surface
quartz crystal microbalance
fluorescence
enzyme-linked immunosorbent assay
biosensor
PMIP

Software Mentioned

Optogenesys
Excel

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