Potyviral Gene-Silencing Suppressor HCPro Interacts with Salicylic Acid (SA)-Binding Protein 3 to Weaken SA-Mediated Defense Responses

Molecular Plant-microbe Interactions : MPMI
Sylvain PoqueShyi-Dong Yeh

Abstract

The viral infection process is a battle between host defense response and pathogen antagonizing action. Several studies have established a tight link between the viral RNA silencing suppressor (RSS) and the repression of salicylic acid (SA)-mediated defense responses, nonetheless host factors directly linking an RSS and the SA pathway remains unidentified. From yeast two-hybrid analysis, we identified an interaction between the potyviral RSS helper-component proteinase (HCPro) and SA-binding protein SABP3. Co-localization and bimolecular fluorescence complementation analyses validated the direct in vivo interaction between Turnip mosaic virus (TuMV) HCPro and the Arabidopsis homologue of SABP3, AtCA1. Additionally, transient expression of TuMV HCPro demonstrated its ability to act as a negative regulator of AtCA1. When the plants of the AtCA1 knockout mutant line were inoculated with TuMV, our results indicated that AtCA1 is essential to restrict viral spreading and accumulation, induce SA accumulation, and trigger the SA pathway. Unexpectedly, the AtCA1 overexpression line also displayed a similar phenotype, suggesting that the constitutive expression of AtCA1 antagonizes the SA pathway. Taken together, our results depict AtCA...Continue Reading

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Citations

Feb 15, 2020·Scientific Reports·Roberta SpanòTiziana Mascia
Nov 20, 2018·Frontiers in Plant Science·Dinesh Babu Paudel, Hélène Sanfaçon
Sep 7, 2019·International Journal of Molecular Sciences·Igor PokotyloEric Ruelland
May 22, 2018·Molecular Plant-microbe Interactions : MPMI·Daniel F KlessigD'Maris Amick Dempsey
Feb 9, 2021·The Plant Pathology Journal·Peter Palukaitis, Su Kim
Feb 9, 2021·Plant, Cell & Environment·Carlos Augusto ManacordaSebastián Asurmendi

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