Potyviral helper-component proteinase expressed in transgenic plants enhances titers of Potato leaf roll virus but does not alleviate its phloem limitation

Virology
Eugene I Savenkov, Jari P T Valkonen

Abstract

Coinfection of Nicotiana benthamiana with Potato virus A (PVA, a potyvirus) and Potato leaf-roll virus (PLRV, a luteovirus) induces a synergistic interaction manifested by enhanced titers of PLRV. The helper component proteinase (HC-Pro) of potyviruses is involved in viral vascular movement and suppression of an antiviral defense mechanism in plants. Data of our study showed that accumulation of PLRV in transgenic N. benthamiana expressing the PVA HC-Pro was enhanced on average by 4.5-fold, as compared to a 6.0-fold enhancement in wild-type N. benthamiana plants doubly infected with PVA and PLRV. Enhancement of PLRV accumulation was directly proportional to the concentration of the HC-Pro in leaves. In the HC-Pro-transgenic plants and wild-type plants, PLRV was almost exclusively confined to the phloem, but the HC-Pro-transgenic plants had a fourfold greater number of PLRV-infected cells within the phloem tissues, as revealed by immunohistochemical staining. In the leaves doubly infected with PLRV and PVA, PLRV was found to exit the phloem in 25.0% of the veins, infecting all types of leaf cells, but, on average, PLRV accumulation was not enhanced more than by sixfold at the whole-leaf level. Therefore, potyviral/luteoviral syn...Continue Reading

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Citations

Aug 16, 2008·Applied Biochemistry and Biotechnology·Pengda MaXingzhi Wang
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·Tefera MekuriaJeanmarie Verchot-Lubicz
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Sep 24, 2019·Phytopathology·Ana Beatriz Moreno, Juan José López-Moya

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