Pouch colon associated with anorectal malformations fails to show spontaneous contractions but responds to acetylcholine and histamine in vitro

Journal of Pediatric Surgery
Preeti TyagiA N Gangopadhyay

Abstract

Congenital pouch colon (CPC) associated with anorectal malformation (ARM) is most commonly reported from Northern India. So far, no physiologic study comparing the detailed contractile status of CPC with non-CPC conditions are available. The present article deals with the contractile study and histopathologic observations in CPC, which may be useful for better surgical management. Freshly excised 12 neonatal CPC and similar number of non-CPC (control) specimens were transferred to ice-cold (4 degrees C-6 degrees C) Krebs-Ringer solution bubbled with 100% oxygen. Longitudinally prepared 2 to 4 colonic strips were obtained from central part of each specimen and subjected to the contraction recording after exposure to cumulative concentrations of acetylcholine (ACh) and histamine. Acetylcholine-induced contractions were evaluated after application of atropine (muscarinic blocker), and histaminergic contractions were recorded after pheniramine (H(1) blocker), lignocaine (neuronal blocker), and atropine. Histopathologic observations were made by using H&E and Masson trichrome stains. Control specimens showed spontaneous contractions, but CPC strips did not. Both control and CPC responded to ACh and histamine. The response to histami...Continue Reading

References

Mar 1, 1980·British Journal of Clinical Pharmacology·B I ReesL E Hughes
Mar 1, 1996·Journal of Pediatric Surgery·A K WakhluS N Kureel
Apr 24, 2004·Pediatric Surgery International·Rajiv Chadha
Mar 24, 2006·The Journal of Pharmacology and Experimental Therapeutics·Weibiao CaoVictor E Pricolo
Jul 26, 2006·Journal of Pediatric Surgery·Archana PuriAnju Garg
Jul 29, 2006·The Journal of Physiology·Kenton M Sanders, Sean M Ward
Mar 24, 2009·Journal of Pediatric Surgery·Ajay Narayan GangopadhyayVijai Dutt Upadhyaya

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Citations

Jul 28, 2013·Pediatric Surgery International·S W MooreK Lakhoo
Jul 30, 2021·Journal of Indian Association of Pediatric Surgeons·Miriam DuciPiergiorgio Gamba

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