Pouteria ramiflora (Mart.) Radlk. extract: Flavonoids quantification and chemopreventive effect on HepG2 cells

Journal of Toxicology and Environmental Health. Part a
Katiuska TuttisIlce Mara de Syllos Cólus

Abstract

Pouteria ramiflora (Mart.) Radlk., popularly known as curriola, is commonly used in Brazil as medicinal plant to treat worm infections, dysentery, pain, inflammation, hyperlipidemia, and obesity. At present the safety of this extract when used therapeutically in human remains to be determined. Thus, the aim of this study was to examine cytotoxicity, antiproliferative, and antimutagenic actions of this extract. The hydroalcoholic extract from P. ramiflora leaves consisted of flavonoids identified and quantified as myricetin-3-O-β-D-galactopyranoside (13.55 mg/g) and myricetin-3-O-α-L-rhamnopyranoside (9.61 mg/g). The extract exhibited cytotoxicity at concentrations higher than 1.5 µg/ml in human hepatocarcinoma (HepG2)and 2.5 µg/ml in non-tumoral primary gastric (GAS) cells using the MTT assay, and at concentrations higher than 3 µg/ml in HepG2 and 3.5 µg/ml in GAS cells by the neutral red assay. The extract did not show antiproliferative effect as evidenced by the nuclear division index (NDI). However, in the presence of benzo[a]pyrene (BaP) (positive control), an enhanced cytostatic effect in the NDI and flow cytometry was noted. It is of interest that when the extract was co-incubated with BaP a significant decrease in DNA da...Continue Reading

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Citations

Feb 12, 2019·Journal of Toxicology and Environmental Health. Part a·Joubert Aires De SousaJaqueline Nascimento Picada
Mar 9, 2019·Journal of Toxicology and Environmental Health. Part a·Tamiris Alves RochaMaria Tereza Dos Santos Correia
May 9, 2019·Journal of Toxicology and Environmental Health. Part a·Nayane Moreira MachadoDenise Crispim Tavares
Jul 19, 2019·Journal of Toxicology and Environmental Health. Part a·Sandra Mara DuavyNilda Vargas Barbosa
Jan 8, 2020·Journal of Toxicology and Environmental Health. Part a·Edson Luis MaistroPaulo Cesar Pires Rosa
May 6, 2020·Journal of Toxicology and Environmental Health. Part a·Fernanda MajoloMárcia Inês Goettert
Jun 23, 2020·Journal of Toxicology and Environmental Health. Part a·Ana Paula Oliveira De QuadrosEdson Luis Maistro
Jun 28, 2020·Journal of Toxicology and Environmental Health. Part a·Higor Lopes NunesIlce Mara de Syllos Cólus
Sep 5, 2020·Journal of Toxicology and Environmental Health. Part a·Karoline Soares de FreitasDenise Crispim Tavares
May 28, 2020·Journal of Toxicology and Environmental Health. Part a·Amanda Cristina CorveloniMário Sérgio Mantovani
Sep 29, 2020·Journal of Toxicology and Environmental Health. Part a·Nathalie Gaebler VasconcelosSimone Simionatto
Oct 2, 2020·Journal of Toxicology and Environmental Health. Part a·Jamilson Dos Santos FreireFrancielle Alline Martins
Oct 27, 2020·Journal of Toxicology and Environmental Health. Part a·Bruno Coêlho CavalcantiManoel Odorico Moraes
Dec 10, 2020·Journal of Toxicology and Environmental Health. Part a·Diego Luis RibeiroLusânia Maria Greggi Antunes
Apr 23, 2021·Environmental Toxicology·Juliana Mara SerpeloniIlce Mara de Syllos Cólus
Jul 15, 2021·Journal of Toxicology and Environmental Health. Part a·Nelzi Ferreira Queiroz JuniorFrancine Carla Cadoná
Aug 14, 2021·Journal of Toxicology and Environmental Health. Part a·Doan Chinh ChungNghia Son Hoang

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Methods Mentioned

BETA
biopsy
Flow
flow cytometry

Software Mentioned

GraphPad Prism
JASCOChrom Nav
Cell Quest
Chrom Nav
Flow Jo

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