Povidone-iodine results in rapid killing of thymic epithelial tumour cells through cellular fixation†

Interactive Cardiovascular and Thoracic Surgery
Hyun-Sung LeeBryan M Burt

Abstract

Hyperthermic pleural lavage with povidone-iodine (PVP-I) is utilized to control micrometastatic disease following cytoreductive surgery for thymic epithelial tumours (TETs). Our objective was to investigate whether PVP-I demonstrates direct cytotoxicity against human TET cells. Human Met-5A (immortalized mesothelial cell), IU-TAB-1 (thymoma) and Ty-82 (thymic carcinoma) cell lines were treated with serial dilutions of PVP-I (0.01-10%) for 5, 30 and 60 min at 37°C and 42°C. MTT assays and flow cytometry were used to evaluate cell death and apoptosis. Membrane permeability was assayed by intracellular staining of cleaved poly-ADP-ribose polymerase. Cellular fixation was evaluated by membrane disruption of dead cells by dimethylsulphoxide and by comparing cleaved poly-ADP-ribose polymerase staining following PVP-I with known fixatives. MTT assays demonstrated that PVP-I concentrations greater than 0.5% led to rapid cell death in both TET cell lines regardless of temperature. IC50 values following 5 min of exposure to PVP-I were 8.4 mM (0.3%) and 13.3 mM (0.48%) for IU-TAB-1 and Ty-82, respectively and 8.9 mM (0.32%) for MeT-5A. Flow cytometry demonstrated that 5-min exposure of either cell line to 1% PVP-I resulted in profound cel...Continue Reading

References

Jan 1, 1997·Dermatology : International Journal for Clinical and Investigative Dermatology·H SchreierW Fleischer
Feb 28, 2002·Journal of Cancer Research and Clinical Oncology·E-M EhlersG J Wiedemann
Sep 10, 2003·The Annals of Thoracic Surgery·Kazuya Kondo, Yasumasa Monden
Sep 17, 2003·The Journal of Cell Biology·Markus RehmJochen H M Prehn
Aug 3, 2007·Interactive Cardiovascular and Thoracic Surgery·Ali YeginsuIlker Etikan
Sep 21, 2007·The New England Journal of Medicine·Lars WagenfeldGisbert Richard
Nov 21, 2007·The Journal of Thoracic and Cardiovascular Surgery·James HuangValerie W Rusch
Nov 3, 2010·The British Journal of Ophthalmology·San-Fang ChouShu-Wen Chang
Dec 22, 2010·Interactive Cardiovascular and Thoracic Surgery·Elizabeth BelcherLoic Lang-Lazdunski
Oct 28, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Ottavio RenaCaterina Casadio
Jan 8, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Alfonso FiorelliMario Santini
Jan 25, 2014·Frontiers in Oncology·Mark Shapiro, Robert J Korst
Mar 25, 2014·The Annals of Thoracic Surgery·Katsuhiro OkudaHisashi Tateyama
Apr 15, 2014·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Tiepeng XiaoShinobu Uehara
May 21, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Tomohiro MurakawaJun Nakajima
Feb 18, 2016·Toxicologic Pathology·Susan A ElmoreDianne M Creasy
Apr 28, 2017·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Bernhard MoserWalter Klepetko
Jul 18, 2017·Translational Lung Cancer Research·Warren Ho ChanBryan M Burt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis