Power consumption and maximum energy dissipation in a milliliter-scale bioreactor

Biotechnology Progress
Ralf Hortsch, Dirk Weuster-Botz

Abstract

Mean power consumption and maximum local energy dissipation were measured as function of operating conditions of a milliliter-scale stirred tank bioreactor (V = 12 mL) with a gas-inducing impeller. A standard laboratory-scale stirred tank bioreactor (V = 1,200 mL) with Rushton turbines was used as reference. The measured power characteristics (Newton number as function of Reynolds number) were the same on both scales. The changeover between laminar and turbulent flow regime was observed at a Reynolds number of 3,000 with the gas-inducing stirrer on a milliliter-scale. The Newton number (power number) in the turbulent flow regime was 3.3 on a milliliter-scale, which is close to values reported for six-blade Rushton turbines of standard bioreactors. Maximum local energy dissipation (epsilon(max)) was measured using a clay/polymer flocculation system. The maximum local energy dissipation in the milliliter-scale stirred tank bioreactor was reduced compared with the laboratory-scale stirred tank at the same mean power input per unit mass (epsilon(ø)), yielding epsilon(max)/epsilon(ø) approximately 10 compared with epsilon(max)/epsilon(ø) approximately 16. Hence, the milliliter-scale stirred tank reactor distributes power more unifor...Continue Reading

References

Jul 29, 2005·Bioprocess and Biosystems Engineering·Dirk Weuster-BotzMatthias Arnold
Nov 24, 2005·Biotechnology and Bioengineering·Peter HarmsGovind Rao
Nov 30, 2005·Biotechnology and Bioengineering·Philip J LeeLuke P Lee
Jan 15, 2008·Bioprocess and Biosystems Engineering·Andreas KustererDirk Weuster-Botz

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Citations

Mar 4, 2010·Biotechnology and Bioengineering·Ralf HortschDirk Weuster-Botz
Sep 18, 2020·Micromachines·Spyridon AchinasGerrit Jan Willem Euverink

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