Power of exclusion of 19 microsatellite markers for parentage testing in river buffalo (Bubalus bubalis).

Molecular Biology Reports
P KathiravanB P Mishra

Abstract

In the present study, 19 microsatellite markers were assessed for their power of exclusion to test parentage in river buffalo. Microsatellite genotypes of 216 unrelated buffaloes belonging to five different breeds were utilized for the study. The probabilities of exclusion were calculated for three hypothetical situations viz. paternity testing (PE1), one parental genotype unavailable (PE2) and exclusion of both parents i.e. substituted offspring (PE3). The mean probability of exclusion across 19 investigated markers in buffalo was 0.578 (PE1), 0.405 (PE2) and 0.764 (PE3) respectively. The probability of exclusion for paternity (PE1) ranged between 0.297 and 0.814 across different markers. The exclusion probability for the cases one parent unavailable (PE2) and substituted offspring (PE3) varied from 0.143 to 0.688 and 0.465 to 0.946 respectively. Polymorphism information content and expected heterozygosity were found to have significantly high correlation with probability of exclusion of microsatellite markers. The cumulative PE1 of nine marker loci was estimated to be 0.9999 while in case of absence of one of the parental genotypes, a minimum of 11 markers were required to achieve a cumulative PE2 of 0.999. In conclusion, the...Continue Reading

References

Jun 1, 1994·Nature Genetics·G GyapayJ Weissenbach
May 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·H Ellegren
Aug 1, 1993·Human Molecular Genetics·J L Weber, C Wong
Jun 6, 1998·Animal Genetics·A Jamieson, S C Taylor
Oct 26, 2001·Research in Veterinary Science·M V ArrugaR Ponz
Sep 13, 2003·Molecular Ecology·Adam G Jones, William R Ardren
Jan 26, 2006·Journal of Veterinary Science·Sun-young Lee, Gil-jae Cho
Feb 28, 2006·Molecular Ecology·Satish KumarMuniyandi Nagarajan
Mar 18, 2008·Journal of Animal Science·R K VijhS T Bharani Kumar
Feb 12, 2009·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·B P MishraD K Sadana
Jul 14, 2011·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·P KathiravanB K Joshi

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Citations

May 1, 2021·Animals : an Open Access Journal From MDPI·Emel Özkan ÜnalMehmet İhsan Soysal

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