Powerful usage of phylogenetically diverse Staphylococcus aureus control strains for detecting multidrug resistance genes in transcriptomics studies

Molecules and Cells
Jun-Sang HamHeebal Kim

Abstract

Staphylococcus aureus is an important human pathogen responsible for life-threatening septicemia, endocarditis, and toxic shock syndrome. Although positive (MRSA; ATCC 33591) and negative (MSSA; ATCC 25923) control strains have been used for various pathogenesis or assay studies, little is known about the genomic structure of the strains, and there has been little genome-wide expression analysis. Phylogenetic analyses revealed that ATCC 33591 and ATCC 25923 are the most genetically diverse strains of the 15 S. aureus genomes studied. Microarray analysis showed that the most significantly upregulated group of MRSA genes was the transport group, which includes ATP-binding cassette (ABC) transporters, the two-component system, and the phosphotransferase system. Analysis of the KEGG pathway showed that ABC transporters and the two-component system were the most significantly altered in MRSA. Transcriptional profiling showed a clear difference in gene expression between MRSA and MSSA due to the great genetic distance between the two control strains. Therefore, we suggest that use of the two control strains in comparative genomics or transcriptomics studies would facilitate the identification of major genes for drug resistance in S. ...Continue Reading

References

Aug 1, 1996·Computer Applications in the Biosciences : CABIOS·R D Page
Jan 27, 1999·Bioinformatics·D Posada, K A Crandall
Nov 4, 2000·Expert Opinion on Investigational Drugs·M J Macielag, R Goldschmidt
Jan 4, 2001·International Journal of Antimicrobial Agents·W Witte
Jan 24, 2002·Journal of Bioenergetics and Biomembranes·M M Gottesman, S V Ambudkar
Feb 12, 2002·Bioorganic & Medicinal Chemistry·Masayuki Matsushita, Kim D Janda
Apr 10, 2003·The Veterinary Clinics of North America. Food Animal Practice·Philip M Sears, Kate K McCarthy
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Nov 26, 2003·Antimicrobial Agents and Chemotherapy·D Ashley Robinson, Mark C Enright
Feb 13, 2004·Bioinformatics·Laurent GautierRafael A Irizarry
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Matthew T G HoldenJulian Parkhill
Jul 28, 2004·Trends in Microbiology·Jodi A Lindsay, Matthew T G Holden
Jan 6, 2005·The Journal of Infectious Diseases·Christian CespedesFranklin D Lowy
Mar 11, 2005·Emerging Infectious Diseases·J S WeeseD E Low
Sep 6, 2005·The Journal of Antimicrobial Chemotherapy·Anette LoefflerDavid H Lloyd
Oct 12, 2005·The Journal of Hospital Infection·I M Gould
Feb 3, 2006·Functional & Integrative Genomics·Jodi A Lindsay, Matthew T G Holden
Sep 1, 2006·Nature·Roger J P Dawson, Kaspar P Locher
Nov 14, 2006·Annals of Clinical Microbiology and Antimicrobials·Xander W HuijsdensAlbert J de Neeling
Apr 10, 2007·International Journal of Medical Microbiology : IJMM·Andrea JansenGabriele Bierbaum
Aug 7, 2007·Journal of Bacteriology·Jeremy M YarwoodE Peter Greenberg
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Apr 25, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·G Ralph Corey

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Citations

Aug 9, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·O BouchamiM Miragaia
Aug 16, 2011·Current Opinion in Microbiology·Melanie J Filiatrault
Jun 15, 2011·FEMS Immunology and Medical Microbiology·Mireille PruneauFrançois Malouin
Mar 6, 2015·International Journal of Medical Microbiology : IJMM·J A BroadbentF Huygens
Sep 14, 2017·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Rafael AraosErika M C D'Agata

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