Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins

Virology Journal
Jennifer D YoderDennis Hruby

Abstract

Although many vaccinia virus proteins have been identified and studied in detail, only a few studies have attempted a comprehensive survey of the protein composition of the vaccinia virion. These projects have identified the major proteins of the vaccinia virion, but little has been accomplished to identify the unknown or less abundant proteins. Obtaining a detailed knowledge of the viral proteome of vaccinia virus will be important for advancing our understanding of orthopoxvirus biology, and should facilitate the development of effective antiviral drugs and formulation of vaccines. In order to accomplish this task, purified vaccinia virions were fractionated into a soluble protein enriched fraction (membrane proteins and lateral bodies) and an insoluble protein enriched fraction (virion cores). Each of these fractions was subjected to further fractionation by either sodium dodecyl sulfate-polyacrylamide gel electophoresis, or by reverse phase high performance liquid chromatography. The soluble and insoluble fractions were also analyzed directly with no further separation. The samples were prepared for mass spectrometry analysis by digestion with trypsin. Tryptic digests were analyzed by using either a matrix assisted laser de...Continue Reading

References

Feb 1, 1979·Journal of Virology·D E HrubyJ R Kates
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B S SchnierleB Moss
Apr 1, 1992·The Journal of General Virology·E V Koonin, T G Senkevich
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H W ChangB L Jacobs
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Y Ahn, B Moss
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Y Ahn, B Moss
Mar 28, 1991·Nature·K L GuanJ E Dixon
Mar 1, 1991·Virology·G P JohnsonE Paoletti
Apr 1, 1990·Journal of Virology·S S Broyles, B S Fesler
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P D Gershon, B Moss
Nov 1, 1990·Virology·S J GoebelE Paoletti
Oct 1, 1988·Journal of Virology·E G Niles, J Seto
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Aug 1, 1995·Journal of Virology·D RodríguezJ R Rodríguez
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S Lin, S S Broyles
Oct 1, 1993·Virology·G P JohnsonE Paoletti
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Dec 15, 1996·Virology·B O GvakhariaC K Mathews

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Citations

Oct 6, 2006·Bioinformatics·Melissa Da SilvaChris Upton
May 1, 2009·Genome Research·Deborah ByrneChantal Abergel
May 30, 2008·Journal of Virology·Y KuznetsovA McPherson
Jul 4, 2008·Journal of Virology·Himani BishtBernard Moss
Sep 15, 2006·Journal of Virology·Patricia RenestoJean-Michel Claverie
Aug 15, 2008·Journal of Virology·R Jeremy NicholsPaula Traktman
Dec 26, 2008·Journal of Virology·Alastair TealeMichele Barry
Jan 14, 2011·Journal of Virology·Liliana Maruri-AvidalBernard Moss
Apr 13, 2012·Journal of Virology·Roza IzmailyanWen Chang
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Kim Van VlietGrant McFadden
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Karen L Maxwell, Lori Frappier
Dec 17, 2008·Virology Journal·Bethany UngerPaula Traktman
Oct 17, 2007·PLoS Pathogens·J Mauricio Calvo-CalleLawrence J Stern
Feb 11, 2010·Future Microbiology·Jorge Mendez-Rios, Peter Uetz
Nov 3, 2014·Annual Review of Virology·Todd M GrecoIleana M Cristea
May 29, 2010·Journal of Biomedicine & Biotechnology·Yulanda M WilliamsonJohn R Barr
Jun 17, 2008·Current Opinion in Microbiology·Michael J Allen, William H Wilson
Sep 29, 2006·Virology·Wolfgang ReschBernard Moss
Mar 11, 2015·Molecular & Cellular Proteomics : MCP·Nathan P CroftAnthony W Purcell
Jul 13, 2017·Chemical Reviews·Deborah Fass, Colin Thorpe
Aug 26, 2016·Journal of Virology·Tuan NgoPaul David Gershon
Apr 10, 2015·Journal of Virology·Kathleen A BoylePaula Traktman

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Blast
MASCOT

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