Abstract
Cell cycle ends with cytokinesis that is the physical separation of a cell into two daughter cells. For faithful cytokinesis, cells integrate multiple processes, such as actomyosin ring formation, contraction and plasma membrane closure, into coherent responses. Linear actin assembly by formins is essential for formation and maintenance of actomyosin ring. Although budding yeast's two formins, Bni1 and Bnr1, are known to switch their subcellular localization at the division site prior to cytokinesis, the underlying mechanisms were not completely understood. Here, we provide evidence showing that Bnr1 is dephosphorylated concomitant with its release from the division site. Impaired PP1/Glc7 activity delayed Bnr1 release and dephosphorylation, Bni1 recruitment and actomyosin ring formation at the division site. These results suggest the involvement of Glc7 in this regulation. Further, we identified Ref2 as the PP1 regulatory subunit responsible for this regulation. Taken together, Glc7 and Ref2 may have a role in actomyosin ring formation by modulating the localization of formins during cytokinesis.
References
May 15, 1997·The EMBO Journal·H ImamuraY Takai
Mar 11, 1999·Genes & Development·A Bloecher, K Tatchell
Feb 26, 2000·Molecular Biology of the Cell·E A VallenE Bi
Apr 4, 2000·The Journal of Cell Biology·A Bloecher, K Tatchell
Dec 12, 2001·Nature Cell Biology·Marie EvangelistaAanthony Bretscher
Dec 12, 2001·Nature Cell Biology·Isabelle SagotDavid Pellman
Jul 20, 2002·Archives of Biochemistry and Biophysics·Shraddha S NigavekarJohn F Cannon
Jul 23, 2002·Nature Cell Biology·Isabelle SagotDavid Pellman
Jan 17, 2003·Molecular Biology of the Cell·Laura Trinkle-MulcahyAngus I Lamond
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Dec 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yoshikazu OhyaShinichi Morishita
Jun 10, 2006·Science·Satoshi YoshidaDavid Pellman
Mar 9, 2007·Molecular Biology of the Cell·Shawnna M ButteryDavid Pellman
Mar 22, 2007·Annual Review of Biochemistry·Bruce L Goode, Michael J Eck
Mar 18, 2008·Molecular Cell·Eduard NedeaPeter L Nagy
Dec 6, 2008·Science·Julie C CanmanKaren Oegema
Mar 17, 2009·Molecular Cell·David M Virshup, Shirish Shenolikar
Dec 10, 2009·Nature Reviews. Molecular Cell Biology·Melissa A ChesaroneBruce L Goode
Dec 24, 2009·The Biochemical Journal·Jofre Ferrer-DalmauAntonio Casamayor
Apr 20, 2010·Trends in Biochemical Sciences·Mathieu BollenMonique Beullens
Dec 4, 2010·The Journal of Biological Chemistry·Joanna BloomFrederick R Cross
Jul 14, 2011·Nature Reviews. Molecular Cell Biology·Claudia Wurzenberger, Daniel W Gerlich
Aug 10, 2011·Biological Chemistry·Carsten WlokaErfei Bi
Jun 26, 2012·Cell·Keiko KonoDavid Pellman
Jul 19, 2012·Annual Review of Cell and Developmental Biology·Rebecca A GreenKaren Oegema
Aug 25, 2012·Molecular Biology of the Cell·Shawnna M ButteryDavid Pellman
Sep 5, 2012·The Journal of Cell Biology·Ricardo Nunes BastosFrancis A Barr
Jul 24, 2013·The Journal of Cell Biology·Benjamin D AtkinsDavid Pellman
Jan 29, 2014·FEMS Microbiology Reviews·Mithilesh MishraMohan K Balasubramanian