pp125FAK in human melanocytes and melanoma: expression and phosphorylation

Experimental Cell Research
G Scott, H Liang

Abstract

Focal adhesion kinase (pp125FAK) is a nonreceptor tyrosine kinase which colocalizes with integrins to focal contacts, sites where multiple proteins interact to regulate the assembly of the actin cytoskeleton. Autophosphorylation and activation of pp125FAK occur after integrin clustering or cell adhesion to ligands through cognate integrin receptors and are postulated to mediate integrin signaling events. In this report we examined pp125FAK expression and phosphorylation in normal human melanocytes, an adherent human metastatic melanoma cell line (SKMEL28), and a nonadherent human metastatic melanoma cell line (SKMEL1). We show that SKMEL28 cells express constitutively phosphorylated pp125FAK and that pp125FAK phosphorylation in melanocytes is induced by phorbol esters and growth factors present in melanocyte growth medium. Focal adhesion kinase phosphorylation could be enhanced by b1 integrin-activating antibodies in human melanocytes, but not in SKMEL28 cells. In contrast with SKMEL28 cells, constitutive phosphorylation of pp125FAK was not observed in SKMEL1 cells, and incubation with activating b1 integrin antibodies had no effect on pp125FAK phosphorylation. Absence of pp125FAK phosphorylation in SKMEL1 cells was not due to ...Continue Reading

Citations

Jun 1, 1996·Pigment Cell Research·G ScottD Luthra
Oct 30, 2010·Pigment Cell & Melanoma Research·Lindy McClellandGlynis Scott
May 4, 2000·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·C J WittM R Young
Aug 29, 2007·International Journal of Cancer. Journal International Du Cancer·Darren J SmitRichard A Sturm
Dec 22, 1999·Oncogene·K MaungD C Bennett
Feb 24, 2001·Melanoma Research·K AroniP Davaris
Nov 30, 2000·Melanoma Research·D J Easty, D C Bennett
Aug 28, 2003·Médecine sciences : M/S·Jérôme CornillonDenis Guyotat
Dec 17, 1998·The American Journal of Dermatopathology·S F Cramer
Jun 1, 1996·Journal of Cellular Physiology·M OkaM Ichihashi
Jun 7, 1996·Biochimica Et Biophysica Acta·R K Malik, J T Parsons
Feb 1, 1997·The Journal of Investigative Dermatology·G ScottA Busacco

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