PPARγ Controls Ectopic Adipogenesis and Cross-Talks with Myogenesis During Skeletal Muscle Regeneration

International Journal of Molecular Sciences
Gabriele DammoneJerome N Feige

Abstract

Skeletal muscle is a regenerative tissue which can repair damaged myofibers through the activation of tissue-resident muscle stem cells (MuSCs). Many muscle diseases with impaired regeneration cause excessive adipose tissue accumulation in muscle, alter the myogenic fate of MuSCs, and deregulate the cross-talk between MuSCs and fibro/adipogenic progenitors (FAPs), a bi-potent cell population which supports myogenesis and controls intra-muscular fibrosis and adipocyte formation. In order to better characterize the interaction between adipogenesis and myogenesis, we studied muscle regeneration and MuSC function in whole body Pparg null mice generated by epiblast-specific Cre/lox deletion (PpargΔ/Δ). We demonstrate that deletion of PPARγ completely abolishes ectopic muscle adipogenesis during regeneration and impairs MuSC expansion and myogenesis after injury. Ex vivo assays revealed that perturbed myogenesis in PpargΔ/Δ mice does not primarily result from intrinsic defects of MuSCs or from perturbed myogenic support from FAPs. The immune transition from a pro- to anti-inflammatory MuSC niche during regeneration is perturbed in PpargΔ/Δ mice and suggests that PPARγ signaling in macrophages can interact with ectopic adipogenesis an...Continue Reading

References

Jul 12, 2002·Developmental Cell·Lluis FajasJohan Auwerx
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·A MAURO
Nov 20, 2003·Nature Medicine·Andrea L HevenerJerrold Olefsky
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Takeshi ImaiDaniel Metzger
Dec 4, 2004·The Journal of Clinical Investigation·Andrew C LiChristopher K Glass
Apr 9, 2005·The American Journal of Clinical Nutrition·Dympna GallagherTamara B Harris
May 9, 2007·The Journal of Experimental Medicine·Ludovic ArnoldBénédicte Chazaud
May 10, 2007·Molecular and Cellular Biochemistry·Akira Wagatsuma
Jun 4, 2008·Annual Review of Biochemistry·Peter Tontonoz, Bruce M Spiegelman
Sep 10, 2009·American Journal of Physiology. Endocrinology and Metabolism·Roberto VettorGiovanni Federspil
Nov 7, 2009·Disease Models & Mechanisms·David B Savage
Jan 19, 2010·Nature Cell Biology·Aaron W B JoeFabio M V Rossi
May 12, 2010·Biochemical and Biophysical Research Communications·Didier F PisaniClaude A Dechesne
May 29, 2010·Circulation Research·Ajay Chawla
Sep 3, 2010·Endocrinology·Karim NadraBéatrice Desvergne
Oct 12, 2011·The Journal of Cell Biology·Eusebio PerdigueroPura Muñoz-Cánoves
Nov 2, 2011·Skeletal Muscle·Alison R AngioneShihuan Kuang
Jan 26, 2012·Development·Susan M Abmayr, Grace K Pavlath
Sep 20, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Sanjum P SamaghBrian T Feeley
Dec 1, 2012·Current Opinion in Clinical Nutrition and Metabolic Care·Maya S Bassil, Réjeanne Gougeon
Feb 7, 2013·The FEBS Journal·Marielle SaclierBénédicte Chazaud
Mar 14, 2013·Development·Barbara A Schmidt, Valerie Horsley
Sep 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia von MaltzahnMichael A Rudnicki
Nov 16, 2013·EMBO Reports·C Florian BentzingerMichael A Rudnicki
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Giacomo DonatiFiona M Watt
Apr 30, 2015·American Journal of Physiology. Endocrinology and Metabolism·Preeti ChandrashekarRavi Kambadur
Apr 24, 2016·Molecular Aspects of Medicine·Thomas BriocheAngèle Chopard
May 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tamas VargaBénédicte Chazaud

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Citations

Oct 5, 2019·Animal : an International Journal of Animal Bioscience·X LiM Du
May 3, 2020·The Journal of Physiology·Nicole K BiltzGretchen A Meyer
Jan 17, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Yuni Susanti PratiwiUnang Supratman
Apr 13, 2019·Cellular and Molecular Life Sciences : CMLS·Manuel SchmidtJulia von Maltzahn
Sep 10, 2019·Frontiers in Physiology·Beatrice BiferaliLuca Madaro
Feb 13, 2020·Frontiers in Cell and Developmental Biology·Nicolas CollaoMichael De Lisio
Mar 18, 2021·Journal of Biochemistry·Alaa ElgaabariMako Nakamura

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Methods Mentioned

BETA
flow cytometry
flow-cytometry
FACS
PCRs

Software Mentioned

GraphPad Prism
ImageXpress
ASW FL
VS
MetaXpress

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