PMID: 9555897Apr 29, 1998Paper

PPi-dependent phosphofructokinase from Thermoproteus tenax, an archaeal descendant of an ancient line in phosphofructokinase evolution

Journal of Bacteriology
B SiebersR Hensel

Abstract

Flux into the glycolytic pathway of most cells is controlled via allosteric regulation of the irreversible, committing step catalyzed by ATP-dependent phosphofructokinase (PFK) (ATP-PFK; EC 2.7.1.11), the key enzyme of glycolysis. In some organisms, the step is catalyzed by PPi-dependent PFK (PPi-PFK; EC 2.7.1.90), which uses PPi instead of ATP as the phosphoryl donor, conserving ATP and rendering the reaction reversible under physiological conditions. We have determined the enzymic properties of PPi-PFK from the anaerobic, hyperthermophilic archaeon Thermoproteus tenax, purified the enzyme to homogeneity, and sequenced the gene. The approximately 100-kDa PPi-PFK from T. tenax consists of 37-kDa subunits; is not regulated by classical effectors of ATP-PFKs such as ATP, ADP, fructose 2,6-bisphosphate, or metabolic intermediates; and shares 20 to 50% sequence identity with known PFK enzymes. Phylogenetic analyses of biochemically characterized PFKs grouped the enzymes into three monophyletic clusters: PFK group I represents only classical ATP-PFKs from Bacteria and Eucarya; PFK group II contains only PPi-PFKs from the genus Propionibacterium, plants, and amitochondriate protists; whereas group III consists of PFKs with either cos...Continue Reading

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Citations

Mar 12, 2002·FEMS Microbiology Letters·Takafumi ArimotoTadamichi Takehara
May 5, 2005·The Biochemical Journal·Hatim AhmedBettina Siebers
Apr 5, 2001·FEMS Microbiology Letters·S GebhardH W Morgan
Aug 14, 2015·The Journal of Biological Chemistry·Yoko ChibaTomoyoshi Nozaki
Jul 14, 2020·Critical Reviews in Biotechnology·Dominik Kopp, Anwar Sunna
Jun 2, 2001·The Journal of Biological Chemistry·B SiebersC H Verhees
Sep 26, 2000·The Journal of Biological Chemistry·A Chi, R G Kemp
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·B CanbackC G Kurland
Mar 7, 2014·Microbiology and Molecular Biology Reviews : MMBR·Christopher BräsenBettina Siebers
Oct 3, 2009·Microbiology·Gino J E BaartDirk E Martens
Nov 22, 2001·Journal of Bacteriology·C H VerheesW M de Vos
Jan 31, 2008·Molecular Genetics and Genomics : MGG·Fabrício Ramon LopesClaudia Marcia Aparecida Carareto
Apr 30, 2019·Frontiers in Microbiology·Patrick HaferkampBettina Siebers
Jun 11, 2019·Frontiers in Microbiology·Jeroen Girwar KoendjbiharieRichard van Kranenburg
Jan 15, 2021·International Journal of Biological Macromolecules·Nisar Ahmed ShakirNaeem Rashid
Jul 25, 2003·The Journal of Experimental Biology·Keith A Webster
Nov 1, 2005·Current Opinion in Microbiology·Bettina Siebers, Peter Schönheit
Sep 23, 2021·Annals of Botany·Laura Kathrine PerbyAngelika Mustroph

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