PPIase is associated with the diversity of conotoxins from cone snail venom glands

Biochimie
Lei WangAn-Long Xu

Abstract

Cone snails are incredibly rich sources of bioactive conopeptides with potential for use in neuroscience research and novel drug development. In order to investigate the synthesis of diversified conopeptides in venom glands, the proteome and peptidome profiles of conus venom were analyzed using HPLC and mass spectrometry. The peptidome profile of the venom components with a molecular weight under 10 kDa showed that the peptides with unique mass values from the venom glands of Conus caracteristicus, Conus lividus and Conus textile are 188, 413 and 265, respectively, and there are 39 overlapping peptides among the three species. Proteome profiling of the components with molecular weights above 10 kDa showed that the most abundant proteins (38.6%) are involved in metabolism and that approximately 6.8% of proteins are involved in protein synthesis, folding and post-translational modification. Among these proteins, PPIase is one protein identified from C. textile based on proteomic analysis. Conus PPIase was successfully expressed as a fusion protein with TRX in an Escherichia coli expression system for further function study. In-vitro enzyme activity assays showed that cone snail PPIase could induce the cis-trans isomerization of t...Continue Reading

References

Jun 13, 1998·The Journal of Biological Chemistry·T D CaldasG Richarme
Aug 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·B M Olivera, L J Cruz
Jun 30, 2001·Trends in Pharmacological Sciences·S Naaby-HansenR Cramer
Jan 13, 2004·Physiological Reviews·Heinrich Terlau, Baldomero M Olivera
Nov 30, 2005·Cellular and Molecular Life Sciences : CMLS·O BuczekB M Olivera
Jun 5, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Lei WangAnlong Xu
Oct 22, 2008·Expert Review of Proteomics·Songping Liang
Dec 20, 2008·Nature Reviews. Drug Discovery·Tadeusz F MolinskiJonel P Saludes
Aug 1, 2009·Natural Product Reports·Reena Halai, David J Craik
Mar 26, 2010·Journal of Proteome Research·Lemmuel L TayoJohn R Yates
Mar 5, 2011·Cell Biochemistry and Function·Thiago Gomes HeckPaulo I Homem de Bittencourt
Sep 15, 2012·Nature Reviews. Microbiology·Michelle D LeachAlistair J P Brown
Sep 18, 2013·Molecular & Cellular Proteomics : MCP·Ai-hua JinPaul F Alewood
Dec 3, 2015·Journal of Experimental & Clinical Cancer Research : CR·Yu ZhangZhihai Peng

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Citations

Jul 6, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Troy WanandyMichael D Wiese
Nov 26, 2020·Marine Drugs·Antonio Trincone

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