Abstract
This study was to explore the effect of protein phosphatase, Mg2+/Mn2+ dependent 1D knockdown on proliferation and apoptosis as well as p38 MAPK/p53 signaling pathway in acute myeloid leukemia. The expression of protein phosphatase, Mg2+/Mn2+ dependent 1D was detected in acute myeloid leukemia cell lines including SKM-1, KG-1, AML-193, and THP-1 cells, and normal bone marrow mononuclear cells isolated from healthy donors. The knockdown of protein phosphatase, Mg2+/Mn2+ dependent 1D was conducted by transfecting small interfering RNA into AML-193 cells and KG-1 cells. The relative messenger RNA/protein expressions of protein phosphatase, Mg2+/Mn2+ dependent 1D were higher in SKM-1, KG-1, AML-193, and THP-1 cells compared with control cells (normal bone marrow mononuclear cells). After transfecting protein phosphatase, Mg2+/Mn2+ dependent 1D small interfering RNA into AML-193 cells and KG-1 cells, both messenger RNA and protein expressions of protein phosphatase, Mg2+/Mn2+ dependent 1D were significantly reduced, indicating the successful transfection. Most importantly, knockdown of protein phosphatase, Mg2+/Mn2+ dependent 1D suppressed cell proliferation and promoted cell apoptosis in AML-193 cells and KG-1 cells. In addition, k...Continue Reading
References
Dec 2, 2000·The EMBO Journal·M TakekawaK Imai
Aug 10, 2006·Breast Cancer Research and Treatment·Eunsil YuJene Choi
Mar 11, 2008·Cancer Genetics and Cytogenetics·Jenita PärssinenAnne Kallioniemi
May 17, 2008·The Journal of Biological Chemistry·Akinori YodaYasuhiro Minami
Dec 26, 2008·Cancer Letters·A RepickyL Cipak
Nov 3, 2009·Trends in Biochemical Sciences·Xavier Le Guezennec, Dmitry V Bulavin
Jul 16, 2010·The Biochemical Journal·Ana Cuadrado, Angel R Nebreda
Dec 18, 2012·Nature·Elise RuarkNazneen Rahman
Dec 22, 2012·Retrovirology·Linda ZaneKuan-Teh Jeang
Sep 17, 2015·The New England Journal of Medicine·Hartmut DöhnerClara D Bloomfield
Dec 31, 2015·Apoptosis : an International Journal on Programmed Cell Death·Bo WuKai-Xing Ai
Jun 9, 2016·The New England Journal of Medicine·Elli PapaemmanuilPeter J Campbell
Jul 2, 2016·Blood Cancer Journal·I De Kouchkovsky, M Abdul-Hay
Nov 11, 2018·Biochemical Pharmacology·Liang-Jun WangLong-Sen Chang
Nov 14, 2018·Nature Genetics·Salam A AssiConstanze Bonifer
Dec 24, 2018·Journal of Biochemistry·Sari OgasawaraKazuyasu Sakaguchi
Mar 11, 2019·Neuro-oncology·Mwangala Precious AkamandisaRobert Craig Castellino
Jul 20, 2019·Therapeutic Advances in Hematology·Eric S Winer, Richard M Stone
Aug 7, 2019·Oncotarget·Hasan MahmudEveline S J M de Bont