(p)ppGpp-dependent and -independent pathways for salt tolerance in Escherichia coli

Journal of Biochemistry
Takefusa TarusawaHyouta Himeno

Abstract

Addition of some kinds of translation inhibitors targeting the ribosome such as kasugamycin to the culture medium as well as removal of a ribosome maturation factor or a ribosomal protein provides Escherichia coli cells with tolerance to high salt stress. Here, we found that another kind of translation inhibitor, serine hydroxamate (SHX), which induces amino acid starvation leading to (p)ppGpp production, also has a similar effect, but via a different pathway. Unlike kasugamycin, SHX was not effective in (p)ppGpp-null mutant cells. SHX and depletion of RsgA, a ribosome maturation factor, had an additive effect on salt tolerance, while kasugamycin or depletion of RsgA did not. These results indicate the presence of two distinct pathways, (p)ppGpp-dependent and -independent pathways, for salt tolerance of E. coli cell. Both pathways operate even in the absence of σ(S), an alternative sigma factor involved in the stationary phase or stress response. Hastened activation of the exocytoplasmic stress-specific sigma factor, σ(E), after salt shock was observed in the cells treated with SHX, as has been observed in the cells treated with a translation inhibitor or depleted of a ribosome maturation factor.

References

Jan 1, 1975·Journal of Supramolecular Structure·V K KalraA F Brodie
Sep 1, 1984·Journal of Bacteriology·A L Koch
Jan 1, 1994·Annual Review of Microbiology·P C Loewen, R Hengge-Aronis
Nov 14, 1997·Journal of Bacteriology·A De Las PeñasC A Gross
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·J M Wood
Dec 14, 1999·Molecular Microbiology·A A Bianchi, F Baneyx
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Mar 17, 2001·Molecular Microbiology·N MajdalaniS Gottesman
Sep 7, 2001·Annual Review of Microbiology·T L Raivio, T J Silhavy
Aug 24, 2002·Journal of Bacteriology·Matthew Hirsch, Thomas Elliott
Apr 22, 2004·Molecular Cell·Shun Jin Lee, Jay D Gralla
Apr 23, 2004·Molecular Microbiology·Benjamin M Alba, Carol A Gross
Dec 13, 2005·PLoS Biology·Virgil A RhodiusCarol A Gross
Sep 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Pohl MilonClaudio O Gualerzi
Jul 10, 2007·Journal of Bacteriology·Mengxi JiangJanine R Maddock
Mar 25, 2008·Current Opinion in Microbiology·Anjana Srivatsan, Jue D Wang
Dec 17, 2008·PLoS Genetics·Daniel J Ferullo, Susan T Lovett
Oct 13, 2009·Journal of Bacteriology·Houra MerrikhSusan T Lovett
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ulrike SchumannIan R Booth
Nov 26, 2010·The EMBO Journal·Simon GotoHyouta Himeno
Jun 7, 2011·Annual Review of Microbiology·Aurelia BattestiSusan Gottesman
Aug 28, 2012·Current Microbiology·Andrew J StasicCharles W Kaspar
Mar 1, 2013·The Journal of Biological Chemistry·Lise GoltermannThomas Bentin
Jul 16, 2013·Critical Reviews in Microbiology·Daniela PintoLélia Chambel
Jul 23, 2013·Applied and Environmental Microbiology·Eveliina PalonenHannu Korkeala
Jan 31, 2015·Current Opinion in Microbiology·Kuanqing LiuJue D Wang

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