PPV long-distance movement is occasionally permitted in resistant apricot hosts

Virus Research
Ligia Ion-NagyVéronique Decroocq

Abstract

The interactions between Plum pox virus (PPV), a member of the Potyvirus genus, and Prunus host plants are, up to now, poorly understood. In the current paper, fluorescence stereomicroscopy, in situ hybridisation and immunogold detection were performed in order to evaluate the virus transport and cellular distribution. The behavior of PPV in several susceptible (cv. "Moniqui" and "Screara") and resistant apricot genotypes (cv. "Harlayne", "Henderson", "Harcot", "Goldrich", "Stella" and "Stark Early Orange") were compared. Viral RNA was detected by in situ hybridisation in stem tissues close to the inoculation point, irrespective of the resistance status of the variety. Systemic infection was evidenced by virus immunodetection and by fluorescence detection of a GFP-tagged PPV in distant leaf sections. The signal obtained by in situ hybridisation colocalised with the fluorescence produced by GFP-tagged PPV in the same plant material but did not colocalise with the signal obtained by immunostaining. Intensity of the PPV infection in susceptible apricot cultivars varied depending on genotypes. The behavior of PPV in systemic leaves was clearly distinct between susceptible and resistant cultivars. While PPV was spreading widely arou...Continue Reading

References

Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·N H ChengR S Nelson
Oct 1, 1996·The Plant Cell·J. C. CarringtonM. C. Schaad
Feb 13, 2003·Molecular Plant-microbe Interactions : MPMI·Minna-Liisa Rajamäki, Jari P T Valkonen
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S. HagenJ.-M. Audergon
Jul 8, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S VilanovaM L Badenes
Jan 25, 2005·Molecular Genetics and Genomics : MGG·V DecroocqJ Kervella
Jul 5, 2005·Journal of Virological Methods·M LansacV Schurdi-Levraud Escalettes

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Citations

Oct 22, 2010·Plant Cell Reports·Elisa Di Nicola-NegriVincenza Ilardi
Jun 23, 2011·Phytopathology·P KogovšekM Pompe-Novak
May 21, 2013·Annual Review of Phytopathology·William O Dawson, Svetlana Y Folimonova
Oct 10, 2013·Molecular Plant Pathology·Juan Antonio GarcíaThierry Candresse
Jul 27, 2007·Virology·Alexey S FolimonovWilliam O Dawson
Oct 7, 2010·Molecular Plant-microbe Interactions : MPMI·Marco A García-Neria, Rafael F Rivera-Bustamante

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