Abstract
The Ki-67 labeling index (LI) is an important prognostic factor in breast carcinoma. The Ki-67 LI is traditionally calculated via unaided microscopic estimation; however, inter-observer and intra-observer variability and low reproducibility are problems with this visual assessment (VA) method. For more accurate assessment and better reproducibility with Ki-67 LI, digital image analysis was introduced recently. We used both VA and automated digital image analysis (ADIA) (Ventana Virtuoso image management software) to estimate Ki-67 LI for 997 cases of breast carcinoma, and compared VA and ADIA results. VA and ADIA were highly correlated (intraclass correlation coefficient 0.982, and Spearman's correlation coefficient 0.966, p<0.05). We retrospectively analyzed cases with a greater than 5% difference between VA and ADIA results. The cause of these differences was: (1) tumor heterogeneity (98 cases, 56.0%), (2) VA interpretation error (32 cases, 18.3%), (3) misidentification of tumor cells (26 cases, 14.9%), (4) poor immunostaining or slide quality (16 cases, 9.1%), and (5) Estimation of non-tumor cells (3 cases, 1.7%). There were more discrepancies between VA and ADIA results in the group with a VA value of 10-20% compared to gro...Continue Reading
References
Jun 1, 1989·British Journal of Cancer·N BouzubarR I Nicholson
Aug 22, 2002·Virchows Archiv : an International Journal of Pathology·F DemichelisS Forti
Apr 25, 2007·British Journal of Cancer·E de AzambujaM Paesmans
May 6, 2008·The Breast : Official Journal of the European Society of Mastology·R Stuart-HarrisP Pharoah
May 14, 2009·Journal of the National Cancer Institute·Maggie C U CheangTorsten O Nielsen
Jun 19, 2009·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A GoldhirschUNKNOWN Panel members
Feb 16, 2010·The Lancet Oncology·Rinat YerushalmiKaren A Gelmon
Apr 22, 2011·Diagnostic Pathology·S FasanellaM Barbareschi
Jun 3, 2011·Breast Care·Michael GnantChristoph Thomssen
Oct 1, 2011·Journal of the National Cancer Institute·Mitch DowsettUNKNOWN International Ki-67 in Breast Cancer Working Group
Jan 19, 2012·British Journal of Cancer·Z M A MohammedJ Edwards
Sep 12, 2012·Histopathology·Einar GudlaugssonJan P A Baak
Oct 3, 2012·The American Journal of Surgical Pathology·Laura H TangDavid S Klimstra
Aug 3, 2013·Cancer Science·Yoshiki MikamiHironobu Sasano
Nov 10, 2013·Pathology Oncology Research : POR·András VörösGábor Cserni
Apr 9, 2014·Breast Cancer Research : BCR·Arvydas LaurinaviciusCatherine Bor
Jun 18, 2014·Diagnostic Pathology·Quinci RomeroSigne Borgquist
Nov 22, 2014·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Michelle D ReidVolkan Adsay
Dec 5, 2014·Pathology·Jennet HarveyGreg Sterrett
Feb 13, 2015·Journal of Clinical Pathology·Akira I HidaTakuya Moriya
May 2, 2015·PloS One·Ruohong ShuiWentao Yang
Jun 14, 2015·Diagnostic Pathology·Patrice DesmeulesVincent Fradet
Jul 25, 2015·Human Pathology·Matthias ChristgenHans Kreipe
Aug 15, 2015·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Ichiro MaedaMasayuki Takagi
Oct 21, 2015·Virchows Archiv : an International Journal of Pathology·Benoit PlancoulaineArvydas Laurinavicius
Jan 29, 2016·Virchows Archiv : an International Journal of Pathology·Arvydas LaurinaviciusIan O Ellis
Feb 16, 2016·Journal of Pathology and Translational Medicine·Yul Ri ChungUNKNOWN Korean Breast Pathology Ki-67 Study Group
Mar 2, 2016·PloS One·Fangfang ZhongRuohong Shui
Apr 20, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Gilbert BigrasHua Yang
Jun 11, 2016·Breast Cancer Research and Treatment·Rasmus RøgeMogens Vyberg
Aug 9, 2016·The Journal of Pathology. Clinical Research·Mustapha AbubakarMontserrat Garcia-Closas
Jun 21, 2017·Indian Journal of Pathology & Microbiology·Lukasz Fulawka, Agnieszka Halon
Jan 20, 2018·Breast Cancer Research and Treatment·Timco KoopmanBert van der Vegt
Citations
Dec 12, 2019·Journal of Medical Imaging·Heba SaadehOmar Al-Kadi
Dec 15, 2019·Journal of Clinical Medicine·Eugeni Lopez-BonetJavier A Menendez
Mar 25, 2020·Annals of Surgical Oncology·Nikolaos A TrikalinosChet Hammill
May 23, 2020·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Rie HoriiFutoshi Akiyama
Nov 27, 2020·Journal of Gastroenterology and Hepatology·Shumpei YamamotoHiroyuki Okada
Jan 13, 2021·JCO Global Oncology·Armand TomaSarah Nietz
Apr 12, 2021·Histopathology·Lijing CaiYueping Liu
Feb 17, 2021·Histopathology·Anna C S BodénDarren Treanor
Apr 14, 2021··Martin LindvallJonas Löwgren