Practical problems encountered during the cultivation of an open-source reconstructed human epidermis model on a polycarbonate membrane and protein quantification

Skin Pharmacology and Physiology
M LemperV Rogiers

Abstract

During recent years, the importance of in vitro technology in skin research has increased significantly. A variety of skin culture models have been developed and commercialized. In this respect, the availability of reconstructed human epidermis (RHE) equivalents represents a significant improvement compared to the use of monolayer cultures. However, when an in-house RHE model is being developed, researchers might encounter some difficulties during cultivation. The scope of this paper is to report our experiences and practical problems with the development of a three-dimensional RHE model cultured on a polycarbonate membrane. Some important issues including cell density, the use of lysing enzymes, culture media, cell storage and viability, cell confluency and protein extraction are reported and optional solutions are given.

Citations

Jul 21, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·F GroeberK R Mewes
Aug 12, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jee-Hyun HwangKyung-Min Lim
Dec 20, 2020·Nanomaterials·Denisse Esther Mallaupoma CamarenaLuiz Henrique Catalani
Jun 2, 2021·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chih-Ching LiaoWannhsin Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Zhongliang LiLiang Chen
Dermatology : International Journal for Clinical and Investigative Dermatology
Mieko NagasawaTakemi Nakayoshi
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
Nobuyuki YamamotoMasakazu Katoh
© 2021 Meta ULC. All rights reserved