Practical Use of RNA Interference: Oral Delivery of Double-stranded RNA in Liposome Carriers for Cockroaches

Journal of Visualized Experiments : JoVE
Jia-Hsin HuangHow-Jing Lee

Abstract

RNA interference (RNAi) has been widely applied for uncovering the biological functions of numerous genes, and has been envisaged as a pest control tool operating by disruption of essential gene expression. Although different methods, such as injection, feeding, and soaking, have been reported for successful delivery of double-stranded RNA (dsRNA), the efficiency of RNAi through oral delivery of dsRNA is highly variable among different insect groups. The German cockroach, Blattella germanica, is highly sensitive to the injection of dsRNA, as shown by many studies published previously. The present study describes a method to demonstrate that the dsRNA encapsulated with liposome carriers is sufficient to retard the degradation of dsRNA by midgut juice. Notably, the continuous feeding of dsRNA encapsulated by liposomes significantly reduces the tubulin expression in the midgut, and led to the death of cockroaches. In conclusion, the formulation and utilization of dsRNA lipoplexes, which protect dsRNA against nucleases, could be a practical use of RNAi for insect pest control in the future.

Citations

Sep 11, 2019·Pest Management Science·Md Abdullah Al BakiYonggyun Kim
Aug 17, 2019·Pest Management Science·Nitish KunteLuz-Adriana Avila
Apr 26, 2021·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·José Carlos MontañésJosé Luis Maestro

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