PMID: 10623749Jan 7, 2000

pRB-dependent, J domain-independent function of simian virus 40 large T antigen in override of p53 growth suppression

Journal of Virology
O GjoerupT M Roberts

Abstract

Simian virus 40 (SV40) large T antigen (LT) can immortalize and transform many cell types. These activities are attributed in large part to the binding and functional inactivation by LT of two major tumor suppressors: p53 and the retinoblastoma protein, pRB. Most effects of LT on pRB have been shown to additionally require an intact J domain, which mediates binding to Hsc70. We show here that the J domain is not required for p53 override in full-length LT. Although LT binds p53, it was shown previously that overcoming a p53-induced cell cycle arrest requires binding to pRB family members (R. S. Quartin et al., J. Virol. 68:1334-1341). We demonstrate that an LT mutant defective for pRB family member binding (K1) can be complemented for efficient override of p53 arrest by a construct encoding the first 135 amino acids of LT with a J domain-inactivating mutation, H42Q. Hence, complementation does not require the J domain, and pRB binding by LT is important for more than dissociating pRB-E2F complexes, which is J dependent. In accordance with this notion, LT alleviates pRB small-pocket-mediated transcriptional repression independently of the J domain. The LT K1 mutant can also be complemented for p53 override by small t antigen (st...Continue Reading

References

Mar 15, 1979·Nature·D P Lane, L V Crawford
Jan 1, 1992·Annual Review of Biochemistry·E Fanning, R Knippers
Apr 1, 1991·Molecular and Cellular Biology·S I YangM Mumby
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Sep 1, 1981·Journal of Virology·E HarlowN M Williamson
Sep 1, 1995·Genes & Development·P SabbatiniE White
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·M L AgarwalG R Stark
May 5, 1995·Cell·Robert A Weinberg
Jul 1, 1994·Trends in Biochemical Sciences·W L Kelley, S J Landry
Sep 1, 1994·Molecular and Cellular Biology·J A FrostJ R Feramisco
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·G W DemersD A Galloway
May 27, 1994·Biochimica Et Biophysica Acta·J J Manfredi, C Prives
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·R J SlebosK R Cho
Nov 19, 1993·Cell·W S El-DeiryB Vogelstein
Dec 1, 1995·Molecular and Cellular Biology·G Del SalC Schneider
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·W R SellersWilliam G Kaelin
May 1, 1996·Genes & Development·L J Ko, C Prives
Jul 1, 1996·Molecular and Cellular Biology·R EcknerJ A Morgan
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·G WatanabeR G Pestell
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·K K Walker, A J Levine
May 1, 1997·Genes & Development·K S CampbellJames A DeCaprio
Jun 1, 1997·Genes & Development·J LukasJ Bartek
Jun 19, 1997·Nature·N L LillD M Livingston
Jan 28, 1998·Oncogene·O MorM Fried
Mar 6, 1998·Molecular and Cellular Biology·J ZalvideJames A DeCaprio
Oct 9, 1998·Genes & Development·A J Giaccia, M B Kastan
Oct 9, 1998·Genes & Development·C J Sherr
Nov 11, 1998·Molecular Cell·S R GrossmanD M Livingston
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M L AgarwalG R Stark
May 14, 1999·Trends in Biochemical Sciences·A Brehm, T Kouzarides

Citations

May 9, 2000·Journal of Virology·Q ShengB Schaffhausen
Sep 5, 2001·Journal of Virology·O GjoerupT M Roberts
Sep 2, 2009·Molecular and Cellular Biology·Yoji Andrew MinamishimaWilliam G Kaelin
Mar 9, 2002·Molecular and Cellular Biology·William C HahnRobert A Weinberg
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Christopher S Sullivan, James M Pipas
Jan 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Marina CotsikiOle V Gjoerup
Apr 9, 2013·The Journal of Investigative Dermatology·Roland HoubenSonja Hesbacher
Jan 5, 2005·Experimental Neurology·William J FreedMary Ellen Truckenmiller
Dec 17, 2008·Virology·James M Pipas
May 6, 2016·Cell Stress & Chaperones·Chetana Ajit Tamadaddi, Chandan Sahi
Dec 10, 2003·Oncogene·Shinichi TakayamaSachiko Homma

Related Concepts

Simian Sarcoma Virus Proteins
Cell Cycle
Cell Division Phases
Cell Transformation, Viral
Cis-Trans Test
Tumor Cells, Cultured
Protein p53
Retinoblastoma Protein
Mutagenesis Process

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.