Pre- and postoperative cytokine release after in vitro whole blood lipopolysaccharide stimulation and frequent toll-like receptor 4 polymorphisms

Shock
Oliver KumpfRalf R Schumann

Abstract

In a prospective cohort study, we examined 62 patients undergoing major surgical cancer therapy for Toll-like receptor 4 (TLR4) gene polymorphisms (Asp299Gly and Thr399Ile) and their influence on cytokine levels pre- and postoperatively, as well as cytokine levels after whole blood lipopolysaccharide (LPS) stimulation. Incidence of the TLR4 gene single nucleotide polymorphism (SNP) Asp299Gly/Thr399Ile was 14.5% (9/62). Overall, mortality was unaffected by the TLR4 SNP. Preoperative cytokine levels were low, with most of the values of cytokines being below the detection levels. After preoperative stimulation of whole blood with 50 pg/mL LPS, TNF-alpha and IL-6 values increased significantly in both groups. However, no significant influence was detectable between the TLR4 SNP group and the wild type group (WT group). Postoperative IL-6 levels, but not TNF-alpha levels, were significantly increased in both groups. Postoperative LPS stimulation resulted in significantly lower TNF-alpha levels compared with preoperative induction, with a more than 2.3-fold decrease in the TLR4 SNP group: 310.83 pg/mL (SD: 117.53) to 134.08 pg/mL (SD: 91.49; P < 0.001) and a 2.2-fold decrease in the WT group: 422.97 pg/mL (SD: 662.57) to 191.68 pg/mL...Continue Reading

References

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Citations

Feb 9, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Tisha S Wang, Jane C Deng
Jul 14, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S N LangerhuusC Lauridsen
Apr 7, 2011·Critical Care : the Official Journal of the Critical Care Forum·Alexander KochKai Zacharowski
Jun 8, 2010·Critical Care : the Official Journal of the Critical Care Forum·Oliver KumpfRalf R Schumann
Jan 31, 2008·Molecular Medicine·Bart FerwerdaMihai G Netea
Oct 15, 2008·Expert Opinion on Therapeutic Targets·Athanasios KatsargyrisStamatios Theocharis
Oct 14, 2008·International Journal of Antimicrobial Agents·Oliver Kumpf, Ralf R Schumann
Dec 19, 2007·Immunobiology·Heike Weighardt, Bernhard Holzmann
Dec 12, 2019·The American Journal of Clinical Nutrition·Nathalie BakkerAlexander P J Houdijk
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Matthew B B McCallRobert W Sauerwein

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