Pre-adsorbed type-I collagen structure-dependent changes in osteoblastic phenotype

Biochemical and Biophysical Research Communications
Nobutaka HanagataJunzo Tanaka

Abstract

Type-I collagen is the most abundant extracellular matrix in bones and modulates various functions of osteoblasts. We prepared two different structures of type-I collagen on tissue culture grade polystylene (TCPS) surfaces, one is feltwork structure of filamentous molecules from acid solutions (ACs) and the other is network structure of fibrils from neutral solutions (NCs), to examine effects of the structures on the maturation process of osteoblast-like cells. No significant differences of cell proliferation were observed between TCPS and ACs, but NCs delayed the proliferation. In initial cell attachment, the cells on ACs had tense lamellipodia with sharp tips, while those on NCs had loose lamellipodia. No detectable differences in levels of expressed integrin alpha2- and alpha5-subunits were observed between the structures. Although the matrix mineralization in NCs was also delayed in comparison with TCPS and ACs, fully mineralized levels in NCs were the same as those of TCPS and ACs. In addition, although we examined the effects of densities of pre-adsorbed collagen molecules on osteoblast maturation, the effects were less serious than those of the structures. This study suggests that the structures of collagen affect prolif...Continue Reading

References

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Citations

Mar 19, 2014·Life Sciences·Kevin DzoboM Iqbal Parker
Jul 14, 2010·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Ricardo OphofJohannes W Von den Hoff
Sep 4, 2008·Journal of Biomedical Materials Research. Part a·Claire M TierneyFergal J O'Brien
Jul 29, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Fu-Yin HsuWei-Bor Tsai
Apr 24, 2007·Journal of Biomedical Materials Research. Part a·Nobutaka HanagataJunzo Tanaka

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