Pre-bled-young-rats in genotoxicity testing: a model for peripheral blood micronucleus assay

Regulatory Toxicology and Pharmacology : RTP
A VikramG B Jena

Abstract

Previously we have reported that prior bleeding increases the sensitivity of micronucleus (MN) assay in rats (Vikram et al., 2007 a). Rat peripheral blood micronucleus (PBMN) assay is generally not considered as reliable method for the assessment of clastogenic potential of test chemicals due to selective elimination of micronucleated cells from the circulation. The present study is aimed to evaluate the sensitivity of pre-bled-young-rat model in detecting genotoxins having different mechanism of action. In the present study, young male Sprague-Dawley (SD) rats (21-24 days old, weighing 60+/-5 g) and swiss mice (24-28 days old, weighing 15+/-2g) were used. Streptozotocin (STZ, 50mg/kg), Methotrexate (MTX, 10mg/kg), N-nitrosodiethylamine (DEN, 200mg/kg), Quercetin (QC, 50mg/kg) and Zidovudine (AZT, 400mg/kg) were used in the present experiment. Effect of prior bleeding time (0, 2, 6, 12 and 24h) on the kinetics of MN formation with STZ and AZT was studied and 36 h post chemical exposure was found to be the most suitable time point for sample collection if prior bleeding time was 0, 2 and 6h. Further, the impact of prior bleeding (2h) on the kinetics of MN formation in the bone marrow was evaluated with STZ and maximum MN frequen...Continue Reading

References

Feb 1, 1975·Mutation Research·W Schmid
Jan 1, 1991·Environmental and Molecular Mutagenesis·J A HeddleJ T MacGregor
Jan 1, 1989·Environmental and Molecular Mutagenesis·Y SuzukiH Shimizu
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J C Li, E Kaminskas
Jun 1, 1994·Mutation Research·M HayashiH Shimada
Aug 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·K M AyersP de Miranda
Nov 24, 1999·Toxicology and Applied Pharmacology·B A DiwanL M Anderson
Aug 5, 2000·Environmental Toxicology and Pharmacology·R C ChoudhuryA K Palo
Jun 18, 2002·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J da SilvaB Erdtmann
May 26, 2005·Mutation Research·Ivonne M C M RietjensGerrit M Alink
Jan 6, 2006·Environmental and Molecular Mutagenesis·Ofelia A Olivero
Mar 16, 2006·Environmental and Molecular Mutagenesis·Ion UdroiuCaterina Tanzarella
Aug 5, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Stephen D DertingerJames T Macgregor
Aug 5, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·James T MacGregorStephen D Dertinger
Feb 20, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J TieppoJ N Picada
Mar 16, 2007·Environmental and Molecular Mutagenesis·Dale M WalkerVernon E Walker
Mar 27, 2007·Biochemical Pharmacology·Gian Luigi Russo
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·J ShugaL G Griffith
Jun 1, 2007·Toxicology and Applied Pharmacology·Agnes W BootsGuido R M M Haenen
Aug 19, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M HarwoodT C Lines
Sep 18, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Susan B HarperJames T MacGregor
Oct 24, 2007·Toxicology and Applied Pharmacology·A VikramG B Jena
Nov 6, 2007·Regulatory Toxicology and Pharmacology : RTP·A VikramG B Jena
Dec 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T García-GascaE González de Mejía
Jan 1, 2004·Environmental Toxicology and Pharmacology·Ramesh C Choudhury, Anil K Palo

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Citations

Feb 27, 2016·Environmental Science and Pollution Research International·Lobna EzziHassen Ben Cheikh
Aug 14, 2020·Journal of Toxicology and Environmental Health. Part a·Daniel Luís Viana CruzMíriam Silva Rafael
Nov 8, 2015·Environmental Science and Pollution Research International·Lobna EzziHassen Ben Cheikh
Mar 24, 2021·Acta Obstetricia Et Gynecologica Scandinavica·Nicolai B JensenLars H Pedersen

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