Pre-Clinical Cell-Based Therapy for Limbal Stem Cell Deficiency

Journal of Functional Biomaterials
Amer SehicTor Paaske Utheim

Abstract

The cornea is essential for normal vision by maintaining transparency for light transmission. Limbal stem cells, which reside in the corneal periphery, contribute to the homeostasis of the corneal epithelium. Any damage or disease affecting the function of these cells may result in limbal stem cell deficiency (LSCD). The condition may result in both severe pain and blindness. Transplantation of ex vivo cultured cells onto the cornea is most often an effective therapeutic strategy for LSCD. The use of ex vivo cultured limbal epithelial cells (LEC), oral mucosal epithelial cells, and conjunctival epithelial cells to treat LSCD has been explored in humans. The present review focuses on the current state of knowledge of the many other cell-based therapies of LSCD that have so far exclusively been explored in animal models as there is currently no consensus on the best cell type for treating LSCD. Major findings of all these studies with special emphasis on substrates for culture and transplantation are systematically presented and discussed. Among the many potential cell types that still have not been used clinically, we conclude that two easily accessible autologous sources, epidermal stem cells and hair follicle-derived stem cell...Continue Reading

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Citations

Mar 10, 2016·Stem Cells and Development·Yas EghtedariNick Di Girolamo
May 25, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ming Shan, Ting-Jun Fan
Mar 5, 2016·Journal of Functional Biomaterials·Tor Paaske UtheimAmer Sehic
Nov 1, 2017·Journal of Cellular Biochemistry·Ebrahim ShirzadehAlireza Baradaran-Rafii
Nov 4, 2017·Investigative Ophthalmology & Visual Science·Liana V BasovaHelen P Makarenkova
Dec 6, 2019·Stem Cells Translational Medicine·Catherine J JacksonTor P Utheim
Jan 16, 2020·International Journal of Molecular Sciences·Joan OlivaYutaka Niihara
Apr 17, 2019·Cellular & Molecular Biology Letters·Chao-Ye DuanMing-Chang Zhang
Mar 11, 2021·The British Journal of Ophthalmology·Praveen ThokalaVirender Singh Sangwan

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Methods Mentioned

BETA
biopsy
flow cytometry
flow
scanning electron microscopy
PCR
scanning
electron microscopy
transgenic

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