Pre-erythrocytic antibody profiles induced by controlled human malaria infections in healthy volunteers under chloroquine prophylaxis

Scientific Reports
Philip L FelgnerRobert W Sauerwein

Abstract

Complete sterile protection to Plasmodium falciparum (Pf) infection mediated by pre-erythrocytic immunity can be experimentally induced under chloroquine prophylaxis, through immunization with sporozoites from infected mosquitoes' bites (CPS protocol). To characterize the profile of CPS induced antibody (Ab) responses, we developed a proteome microarray containing 809 Pf antigens showing a distinct Ab profile with recognition of antigens expressed in pre-erythrocytic life-cycle stages. In contrast, plasma from naturally exposed semi-immune individuals from Kenya was skewed toward antibody reactivity against asexual blood stage antigens. CPS-immunized and semi-immune individuals generated antibodies against 192 and 202 Pf antigens, respectively, but only 60 antigens overlapped between the two groups. Although the number of reactive antigens varied between the CPS-immunized individuals, all volunteers reacted strongly against the pre-erythrocytic antigens circumsporozoite protein (CSP) and liver stage antigen 1 (LSA1). Well classified merozoite and erythrocytic antigens were strongly reactive in semi-immune individuals but lacking in the CPS immunized group. These data show that the antibody profile of CPS-immunized and semi-immu...Continue Reading

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Citations

Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Danica A HelbBryan Greenhouse
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Methods Mentioned

BETA
ELISA
proteome array
in-vitro transcription
chips
Protein Array

Clinical Trials Mentioned

NCT00442377
NCT00757887

Software Mentioned

Bioconductor suite
ProscanArray
ProScanArray Express
QuantArray
Cyber
R

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