Pre-existing technological core and roots for the CRISPR breakthrough

PloS One
Christopher L MageeSubarna Basnet

Abstract

This paper applies objective methods to explore the technological origins of the widely acclaimed CRISPR breakthrough in the technological domain of genome engineering. Previously developed patent search techniques are first used to recover a set of patents that well-represent the genome editing domain before CRISPR. Main paths are then determined from the citation network associated with this patent set allowing identification of the three major knowledge trajectories. The most significant of these trajectories for CRISPR involves the core of genome editing with less significant trajectories involving cloning and endonuclease specific developments. The major patents on the core trajectory are consistent with qualitative expert knowledge of the topical area. A second set of patents that we call the CRISPR roots are obtained by finding the patents directly cited by the recent CRISPR patents along with patents cited by that set of patents. We find that the CRISPR roots contain 8 key patents from the genome engineering main path associated with restriction endonucleases and the expected strong connection of CRISPR to prior genome editing technology such as Zn finger nucleases. Nonetheless, analysis of the full CRISPR roots shows t...Continue Reading

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Citations

Mar 11, 2020·Annual Review of Genomics and Human Genetics·Gregory D Graff, Jacob S Sherkow
Aug 28, 2020·International Journal of Molecular Sciences·Alekya S TanikellaJulia Thom Oxford

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Datasets Mentioned

BETA
US6607882
US5637463

Methods Mentioned

BETA
PCR

Software Mentioned

COM
GBFP
GE2
TALEN

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