Pre-pregnancy maternal obesity associates with endoplasmic reticulum stress in human umbilical vein endothelium

Biochimica Et Biophysica Acta. Molecular Basis of Disease
Roberto Villalobos-LabraLuis Sobrevia

Abstract

Obesity associates with the endoplasmic reticulum (ER) stress-induced endothelial dysfunction. Pregnant women with pre-pregnancy maternal obesity (PGMO) may transfer this potential risk to their offspring; however, whether ER stress occurs and associates with foetoplacental endothelial dysfunction in PGMO is unknown. We studied the l-arginine transport and nitric oxide (NO) synthesis in human umbilical vein endothelial cells (HUVECs) from women with PGMO or with a normal pre-pregnancy weight. We analysed the expression and activation of the ER stress sensors protein kinase RNA-like endoplasmic reticulum kinase (PERK), inositol-requiring enzyme 1α (IRE1α), and activating transcription factor 6 (ATF6). PGMO associated with lower endothelial NO synthase activity due to increased Thr495-inhibitor and decreased Ser1177-stimulator phosphorylation. However, higher expression and activity of the human cationic amino acid transporter 1 was found. PGMO caused activation of PERK and its downstream targets eukaryotic initiation factor 2 (eIF2α), C/EBP homologous protein 10 (CHOP), and tribbles-like protein 3 (TRB3). Increased IRE1α protein abundance (but not its phosphorylation or X-box binding protein 1-mRNA splicing) and increased c-Jun ...Continue Reading

Citations

Jan 31, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Luis SilvaLuis Sobrevia
May 11, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Francisca StolzenbachBredford Kerr
Jan 21, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Mario SubiabreLuis Sobrevia
Sep 1, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Luis SobreviaReinaldo Marín
Nov 27, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Luis Sobrevia
May 18, 2021·Frontiers in Cell and Developmental Biology·Babban JeeSubhradip Karmakar
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sagir MustaphaSiti Safiah Mokhtar
Sep 28, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Marcelo CornejoLuis Sobrevia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.