PMID: 7520961Jul 1, 1993Paper

Pre-stimulation of the human bone marrow CD34+ cells before storage at 4 degrees C with the early acting cytokines enhance their survival and increase proliferative potential. Transplantological implications

Materia Medica Polona. Polish Journal of Medicine and Pharmacy
M Z RatajczakJ Ratajczak

Abstract

The aim of this study was to evaluate whether short prestimulation of the human bone marrow CD34+ cells before storage at 4 degrees C with early acting cytokines would improve their subsequent survival and clonogenecity. To address this question the cells were placed in iscove medium supplemented with 20% BCS and stimulated for 24 hours in 37 degrees C incubator with different combination of the early acting cytokines (KL, IL-1 beta, IL-3, IL-6). Subsequently the cells were shifted to 4 degrees C. On day 6 the cells were plated in methylcelulose cultures and stimulated to growth CFU-GM colonies. It had been found that 24 hours long pre-stimulation with early acting cytokines significantly enhances the survival of the CD34+ clonogeneic progenitors stored subsequently at 4 degrees C. The CD34+ cell prestimulated for 24 hours at 37 degrees C with KL + IL-3 and than shifted to 4 degrees C formed more than twice as much CFU-GM colonies in comparison to the cells stored whole time at 4 degrees C even in the presence of KL + IL-3. Therefore we suggest, that if there is a need to store bone marrow cells before transplantation for short time they should be prestimulated with early acting cytokines for 24 hours in liquid culture at 37 de...Continue Reading

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