Pre-symptomatic diagnosis and treatment of filovirus diseases

Frontiers in Microbiology
Amy C ShurtleffSina Bavari

Abstract

Filoviruses are virulent human pathogens which cause severe illness with high case fatality rates and for which there are no available FDA-approved vaccines or therapeutics. Diagnostic tools including antibody- and molecular-based assays, mass spectrometry, and next-generation sequencing are continually under development. Assays using the polymerase chain reaction (PCR) have become the mainstay for the detection of filoviruses in outbreak settings. In many cases, real-time reverse transcriptase-PCR allows for the detection of filoviruses to be carried out with minimal manipulation and equipment and can provide results in less than 2 h. In cases of novel, highly diverse filoviruses, random-primed pyrosequencing approaches have proved useful. Ideally, diagnostic tests would allow for diagnosis of filovirus infection as early as possible after infection, either before symptoms begin, in the event of a known exposure or epidemiologic outbreak, or post-symptomatically. If tests could provide an early definitive diagnosis, then this information may be used to inform the choice of possible therapeutics. Several exciting new candidate therapeutics have been described recently; molecules that have therapeutic activity when administered ...Continue Reading

References

Oct 1, 1992·The Journal of Infectious Diseases·S P Fisher-HochC S Goldsmith
Nov 1, 1985·The Journal of Infectious Diseases·S P Fisher-HochD I Simpson
Mar 26, 1968·Deutsche medizinische Wochenschrift·R SiegertW Slenczka
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·A SanchezS T Nichol
Feb 13, 1999·The Journal of Infectious Diseases·R F SadekT G Ksiazek
Feb 13, 1999·The Journal of Infectious Diseases·E I RyabchikovaS V Luchko
Jun 28, 2000·Nucleic Acids Research·T NotomiT Hase
May 9, 2002·Emerging Infectious Diseases·Thomas W GeisbertPeter B Jahrling
Feb 24, 2006·Antimicrobial Agents and Chemotherapy·Sven EnterleinElke Mühlberger
Sep 16, 2006·Chembiochem : a European Journal of Chemical Biology·Darryl FalzaranoHeinz Feldmann
Nov 3, 2006·Journal of Clinical Microbiology·Junhui ZhaiW Ian Lipkin
Dec 30, 2006·Journal of Virological Methods·Yohei KurosakiJiro Yasuda
Jan 24, 2007·PLoS Pathogens·Heinz FeldmannThomas W Geisbert
Apr 13, 2007·Journal of Virology·Alexander BukreyevAnthony Sanchez
Dec 6, 2007·The Journal of Infectious Diseases·Karen L Hutchinson, Pierre E Rollin
Dec 6, 2007·The Journal of Infectious Diseases·Lisa E HensleyThomas W Geisbert
Dec 6, 2007·The Journal of Infectious Diseases·Kelly L WarfieldSina Bavari
Nov 11, 2008·Current Pharmaceutical Design·David A Stein
Nov 22, 2008·PLoS Pathogens·Jonathan S TownerStuart T Nichol
Feb 19, 2009·Antimicrobial Agents and Chemotherapy·Dana L SwensonSina Bavari
Feb 25, 2009·Critical Care Medicine·Benjamin M P TangRuby C Y Lin
Apr 9, 2010·Science Translational Medicine·Aimee K ZaasGeoffrey S Ginsburg
Jun 2, 2010·Clinics in Laboratory Medicine·Amy L HartmanStuart T Nichol
Jul 1, 2010·Emerging Infectious Diseases·Thomas W GeisbertHeinz Feldmann

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Citations

Oct 30, 2016·International Journal of Molecular Sciences·Muhammad Usman Mirza, Nazia Ikram
Feb 21, 2019·Proceedings of the National Academy of Sciences of the United States of America·Derin SevenlerM Selim Ünlü
Dec 14, 2016·Annual Review of Pharmacology and Toxicology·Anthony P CardileSina Bavari

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
nucleic acid sequencing

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