Pre-synaptic and post-synaptic localization of EphA4 and EphB2 in adult mouse forebrain

Journal of Neurochemistry
David BouvierGuy Doucet

Abstract

The ephrin receptors EphA4 and EphB2 have been implicated in synaptogenesis and long-term potentiation in the cerebral cortex and hippocampus, where they are generally viewed as post-synaptic receptors. To determine the precise distribution of EphA4 and EphB2 in mature brain synapses, we used subcellular fractionation and electron microscopy to examine the adult mouse forebrain/midbrain. EphA4 and EphB2 were both enriched in microsomes and synaptosomes. In synaptosomes, they were present in the membrane and the synaptic vesicle fractions. While EphA4 was tightly associated with PSD-95-enriched post-synaptic density fractions, EphB2 was easily extracted with detergents. In contrast, both receptors were found in the pre-synaptic active zone fraction. By electron microscopy, EphA4 was mainly detected in axon terminals, whereas EphB2 was more frequently detected in large dendritic shafts, in the hippocampus and cerebral cortex. However, in the ventrobasal thalamus, EphB2 was detected most frequently in axon terminals and thin dendritic shafts. The localization of EphA4 and EphB2 in multiple compartments of neurons and synaptic junctions suggests that they interact with several distinct scaffolding proteins and play diverse roles at...Continue Reading

References

Jan 25, 1996·Nature·P S McPhersonP De Camilli
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·M DottoriA W Boyd
Aug 10, 2000·The Journal of Cell Biology·T W HarrisE M Jorgensen
Mar 15, 2001·Current Opinion in Cell Biology·R Klein
Oct 3, 2001·Neuron·I M EthellY Yamaguchi
Jun 8, 2002·Science·Anis ContractorStephen F Heinemann
Dec 14, 2002·The International Journal of Biochemistry & Cell Biology·Juha-Pekka Himanen, Dimitar B Nikolov
Dec 24, 2002·Nature Neuroscience·Keith K MuraiElena B Pasquale
Nov 19, 2003·Neuron·Kimberly R SchuskeErik M Jorgensen
Dec 24, 2003·The Journal of Cell Biology·Mark HenkemeyerIryna M Ethell
Dec 31, 2003·Nature Neuroscience·Ilona C GrunwaldRüdiger Klein
Apr 27, 2004·Nature Neuroscience·Juha-Pekka HimanenDimitar B Nikolov
Jun 15, 2004·Current Opinion in Neurobiology·Yu Yamaguchi, Elena B Pasquale
Sep 15, 2004·Current Opinion in Cell Biology·Rüdiger Klein
Jun 21, 2005·Nature Neuroscience·Casper C HoogenraadMorgan Sheng
Jul 12, 2005·Neurochemistry International·P S PinheiroJ O Malva
Aug 23, 2005·Brain Research. Brain Research Reviews·Albert Martínez, Eduardo Soriano
Feb 10, 2006·Developmental Biology·Alma Rodenas-RuanoDaniel J Liebl
Mar 21, 2006·Current Biology : CB·Dion K DickmanThomas L Schwarz
Mar 31, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John N ArmstrongAnis Contractor
Jun 16, 2006·Brain Research Reviews·Yona GoldshmitAnn Turnley
Feb 15, 2007·The Journal of Comparative Neurology·Marie-Eve TremblayGuy Doucet
May 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lei ZhouKeith K Murai
Nov 23, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael W NestorScott M Thompson

❮ Previous
Next ❯

Citations

Apr 25, 2012·Acta Neuropathologica·Naoya AokiHaruhiko Akiyama
Jan 15, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Vadim YuferovMary Jeanne Kreek
Apr 22, 2011·Nature·Benjamin K AttwoodRobert Pawlak
Sep 17, 2013·Nature Neuroscience·Anna B TothHisashi Umemori
Apr 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew C McClellandMatthew B Dalva
Jan 15, 2011·The Journal of Biological Chemistry·Stephanie FultonLouis-Eric Trudeau
May 26, 2009·Neural Development·Timothy P O'ConnorShernaz X Bamji
May 6, 2011·Neural Development·Meredith A CliffordMaria J Donoghue
Mar 3, 2011·PloS One·Matthew S KayserMatthew B Dalva
Apr 8, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mark J NoltMatthew B Dalva
Mar 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yasunobu Murata, Martha Constantine-Paton
Aug 7, 2010·Journal of Visualized Experiments : JoVE·Marie-Eve TremblayAnia Majewska
Jul 22, 2014·Behavioural Brain Research·Martin HadamitzkyManfred Schedlowski
Apr 21, 2012·Trends in Neurosciences·Sean I Sheffler-Collins, Matthew B Dalva
Mar 28, 2012·Molecular and Cellular Neurosciences·Martin Hruska, Matthew B Dalva
Jan 24, 2012·The International Journal of Biochemistry & Cell Biology·Benjamin K AttwoodRobert Pawlak
Nov 2, 2011·Seminars in Cell & Developmental Biology·Slawomir Sloniowski, Iryna M Ethell
Dec 17, 2008·The Journal of Comparative Neurology·Marie-Eve TremblayGuy Doucet
Nov 20, 2009·The Journal of Comparative Neurology·Takenori KotaniTakashi Matozaki
Jan 14, 2010·Journal of Neurochemistry·David BouvierGuy Doucet
Jul 17, 2014·Acta Neuropathologica Communications·Andrea Fn RosenbergerJeroen Jm Hoozemans
Jul 15, 2015·Neurobiology of Learning and Memory·Monica DinesRaphael Lamprecht
Jun 25, 2015·BioMed Research International·Wei HuWei J Chen
Apr 23, 2016·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ruo-Xi ZhangLin Lu
Jan 19, 2016·The Journal of Comparative Neurology·Clara A AmegandjinGuy Doucet
Feb 21, 2018·The International Journal of Neuropsychopharmacology·Martin HadamitzkyManfred Schedlowski
Aug 19, 2011·Glia·Keith K Murai, Elena B Pasquale
Feb 20, 2018·Experimental and Therapeutic Medicine·Jin-Shan YangGang Wu
Jul 22, 2018·Molecular and Cellular Neurosciences·Nathan T Henderson, Matthew B Dalva
Sep 28, 2012·Journal of Cell Science·Lin NingCarl G Gahmberg
Jul 28, 2021·Genome Biology·Amelie BaudOliver Stegle
Aug 4, 2021·Advanced Healthcare Materials·Cara T MotzCheng Zhu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.